Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes
Kirkham, Amy R.; Jardillier, Ludwig E.; Holland, Ross; Zubkov, Mikhail V.; Scanlan, Dave J.. 2011 Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes. Deep Sea Research Part I Oceanographic Research Papers, 58 (7). 733-744. 10.1016/j.dsr.2011.05.004
Full text not available from this repository. (Request a copy)Abstract/Summary
Photosynthetic picoeukaryotes (PPEs) of a size <3 μm can contribute significantly to primary production. Here, PPE community structure was analysed along an extended Ellett Line transect, an area in the North Atlantic well studied by physical oceanographers but largely neglected in the field of microalgal ecology. Distribution patterns of specific PPE classes were determined using dot-blot hybridization analysis, while the taxonomic composition of specific PPE classes was revealed by phylogenetic analysis of plastid 16S rRNA gene sequences. In addition, we performed fluorescent in situ hybridization (FISH) analysis of seawater samples collected along the transect to provide a PCR-independent survey of class level PPE distribution patterns. We found the PPE community was dominated by members of the Prymnesiophyceae, Prasinophyceae and Mamiellophyceae. Interestingly, phylogenetic analysis revealed several novel Prymnesiophyceae and Prasinophyceae phylotypes (with only 85–96% identity to neighbouring sequences) within lineages for which cultured counterparts are unknown.
Item Type: | Publication - Article |
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Digital Object Identifier (DOI): | 10.1016/j.dsr.2011.05.004 |
ISSN: | 09670637 |
Additional Keywords: | Photosynthetic picoeukaryotes; Community structure; Prymnesiophytes; Prasinophytes |
Date made live: | 26 Aug 2011 10:47 +0 (UTC) |
URI: | https://nora.nerc.ac.uk/id/eprint/295759 |
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