Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins

Ho, Thien; Wang, Hui; Pallett, Denise; Dalmay, Tamas. 2007 Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins. FEBS Letters, 581 (2007). 3267-3272.

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Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant Dicer-like (DCL) enzymes. Robust GC bias was observed for TuMV siRNAs versus the virus genome, indicating that DCL was more likely to target GC-rich regions. Furthermore, dicot micro-(mi)RNAs displayed higher GC% than their DCL1 substrate RNAs, implicating that the GC bias may be ancient, therefore may be important for the RNAi technology.

Item Type: Publication - Article
Digital Object Identifier (DOI):
Programmes: CEH Programmes pre-2009 publications > Biodiversity > BD03 The Genetic Basis of Ecological Function
UKCEH and CEH Sections/Science Areas: Hails
ISSN: 0014-5793
Additional Information. Not used in RCUK Gateway to Research.: Copy in Digital Archive
Additional Keywords: siRNA, miRNA, compositional bias, Dicer, Turnip mosaic virus, Brassica juncea
NORA Subject Terms: Botany
Biology and Microbiology
Ecology and Environment
Date made live: 19 Mar 2008 12:26 +0 (UTC)

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