Intercalibration exercise using a stickleback endocrine disrupter screening assay

Allen, Yyvonne T.; Katsiadaki, Ioanna; Pottinger, Tom G.; Jolly, Cecile; Matthiessen, Peter; Mayer, Ian; Smith, Andy; Scott, Alexander P.; Eccles, Paul; Sanders, Matthew B.; Pulman, Kim G. T.; Feist, Stephen. 2008 Intercalibration exercise using a stickleback endocrine disrupter screening assay. Environmental Toxicology and Chemistry, 27 (2). 404-412.

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The Organisation for Economic Cooperation and Development (OECD) is currently validating a short-term fish screening protocol for endocrine disrupters (estrogens, androgens, and their antagonists and aromatase inhibitors), using three core species: fathead minnow, Japanese medaka, and zebrafish. The main endpoints proposed for the first phase of validation of the screen are vitellogenin (VTG) concentration, gross morphology (secondary sexual characteristics and gonado-somatic index), and gonadal histopathology. A similar protocol is concurrently being developed in the United Kingdom using the three-spined stickleback, with identical endpoints to those for the core species and, in addition, a unique androgen-specific endpoint in the form of spiggin (glue protein) induction. To assess the suitability of this species for inclusion in the OECD protocol alongside the core species, an intercalibration was conducted using 17β-estradiol (a natural estrogen) and trenbolone (a synthetic androgen), thus mimicking a previous intercalibration with the core species. All three participating laboratories detected statistically significant increases in VTG in males after 14 d exposure to nominal concentrations of 100 ng/L 17β-estradiol and statistically significant increases in spiggin in females after 14 d exposure to nominal concentrations of 5,000 ng/L trenbolone. The stickleback screen is reliable, possessing both relevant and reproducible endpoints for the detection of potent estrogens and androgens. Further work is underway to assess the relevance and suitability of the screen for weakly acting estrogens, anti-androgens, and aromatase inhibitors.

Item Type: Publication - Article
Digital Object Identifier (DOI):
Programmes: CEH Programmes pre-2009 publications > Water > WA02 Quantifying processes that link water quality and quantity, biota and physical environment > WA02.3 Physico-chemical processes and effects on freshwater biot
CEH Programmes pre-2009 publications > Biogeochemistry > SE01B Sustainable Monitoring, Risk Assessment and Management of Chemicals > SE01.5 Exposure, Effects and Managing Risks of Chemicals
UKCEH and CEH Sections/Science Areas: Parr
ISSN: 0730-7268
Additional Keywords: Stickleback, Intercalibration, Endocrine disrupter, Fish screening assay
NORA Subject Terms: Biology and Microbiology
Ecology and Environment
Date made live: 21 Jan 2008 15:14 +0 (UTC)

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