On the use of the FluoroProbe®, a phytoplankton quantification method based on fluorescence excitation spectra for large-scale surveys of lakes and reservoirs

Catherine, A.; Escoffier, N.; Belhocine, A.; Nasri, A.B.; Hamlaoui, S.; Yéprémian, C.; Bernard, C.; Troussellier, M.. 2012 On the use of the FluoroProbe®, a phytoplankton quantification method based on fluorescence excitation spectra for large-scale surveys of lakes and reservoirs. Water Research, 46 (6). 1771-1784.

Full text not available from this repository.


Although microscope analysis is very useful for studying phytoplankton communitycomposition, it does not allow for high frequency (spatial and/or temporal) data acquisi-tion. In an attempt to overcome this issue, fluorescence-based approaches that useselective excitation of pigment antennae have spread rapidly. However, the ability of spectral fluorescence to provide accurate estimates of phytoplankton biomass andcomposition is still debated, and only a few datasets have been tested to date.In this study, we sampled of a wide range of water bodies (n¼50) in the Ile-de-Franceregion (North Central France). We used the resulting extensive dataset to assess theability of the bbe-Moldaenke FluoroProbe II (FP) to estimate phytoplankton communitycomposition in lakes and reservoirs.We demonstrated that FP data yields better estimates of total phytoplankton biovolumethan do spectrophotometric chlorophyllameasures and that FP data can be further cor-rected using the average chlorophyllato biovolume ratio among phytoplankton groups.Overall, group-specific relationships between FP and biovolume data were consistent.However, we identified a number of cases where caution is required. We found thatEuglenophytes are expected to depart from the global FPvs.biovolume relationship of the‘green’ group due to varying Fv/Fm and pigment content in response to environmentalconditions (photoautotrophicvs.photoheterotrophic growth). Then, it appears necessaryto consider the composition of the Chromophytes community in order to obtain a goodagreement between both biomass estimation methods. Finally, we confirmed the misat-tribution toward the ‘red’ group of phycoerythrin-containing cyanobacteria and theoccurrence of a strong scattering in the relationship between the FPvs.biovolume of the‘blue’ group that can be partly attributed to the occurrence of large colony-forming cya-nobacteria (e.g.,Microcystisspp,Aphanizomenon flos-aquae).We propose correcting procedures to improve the quality of data obtained from spectral fluorescence tools in the context of large-scale sampling of lakes and reservoirs.

Item Type: Publication - Article
Digital Object Identifier (DOI):
Programmes: CEH Topics & Objectives 2009 - 2012 > Water
UKCEH and CEH Sections/Science Areas: Parr
ISSN: 0043-1354
Additional Keywords: phytoplankton, community composition, pigment antennae, chlorophyll a, biomass
NORA Subject Terms: Ecology and Environment
Biology and Microbiology
Date made live: 17 Jan 2013 15:17 +0 (UTC)

Actions (login required)

View Item View Item

Document Downloads

Downloads for past 30 days

Downloads per month over past year

More statistics for this item...