Transfer of microsatellite loci for the tropical tree Prunus africana (Hook. f.) Kalkman.

Cavers, S. ORCID: https://orcid.org/0000-0003-2139-9236; Munro, R.C.; Kadu, C.A.C.; Konrad, H.. 2009 Transfer of microsatellite loci for the tropical tree Prunus africana (Hook. f.) Kalkman. Silvae Genetica, 58 (5-6), 10.

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Abstract/Summary

Using databases of previously published primers, we optimised six nuclear microsatellite markers for Prunus africana for the purposes of studying spatial genetic structure and gene flow. To assess variability, these and three previously transferred loci were screened in populations from Kenya and South Africa. Across both populations most loci were polymorphic, with the exception of a single locus which failed to amplify in the South African samples, exhibiting between 2 and 22 alleles and levels of expected heterozygosity (He) ranged from 0.059 to 0.932. Departures from Hardy–Weinberg equilibrium were detected for all loci but not for all populations. The estimated null allele frequency was very low to moderate and no evidence for linkage disequilibrium was detected.

Item Type:	Publication - Article
Programmes:	CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.3 - Identify indicators and traits of ecosystem status ... CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.4 - Estimate the impact of the main drivers and pressures on biodiversity ... CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 3 - Managing Biodiversity and Ecosystem Services in a Changing Environment > BD - 3.4 - Provide science-based advice ...
UKCEH and CEH Sections/Science Areas:	Watt
ISSN:	0037-5349
Additional Keywords:	Prunus africana, nuclear microsatellites, gene flow, genetic diversity
NORA Subject Terms:	Ecology and Environment
Date made live:	22 Feb 2010 16:50 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/7695

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