A sampling strategy for genome sequencing the British terrestrial arthropod fauna

Crowley, Liam; Allen, Heather; Barnes, Ian; Boyes, Douglas ORCID: https://orcid.org/0000-0003-0191-3920; Broad, Gavin R.; Fletcher, Christopher; Holland, Peter W.H.; Januszczak, Inez; Lawniczak, Mara; Lewis, Owen T.; Macadam, Craig R.; Mulhair, Peter O.; Pereira da Conceicoa, Lyndall; Price, Benjamin W.; Raper, Chris; Sivell, Olga; Sivess, Laura. 2023 A sampling strategy for genome sequencing the British terrestrial arthropod fauna. Wellcome Open Research, 8, 123. 16, pp. https://doi.org/10.12688/wellcomeopenres.18925.1

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Official URL: http://dx.doi.org/10.12688/wellcomeopenres.18925.1

Abstract/Summary

The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation. We briefly explore some lessons learned from the pilot phase of DToL and the impact of the Covid-19 pandemic.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.12688/wellcomeopenres.18925.1
UKCEH and CEH Sections/Science Areas:	Biodiversity (Science Area 2017-)
ISSN:	2398-502X
Additional Information. Not used in RCUK Gateway to Research.:	Open Access paper - full text available via Official URL link.
Additional Keywords:	Earth BioGenome Project, invertebrate, whole-genome sequencing, collecting, preservation, prioritisation
NORA Subject Terms:	Ecology and Environment Zoology Data and Information
Date made live:	10 Jul 2023 16:16 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/535346

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.12688/wellcomeopenres.18925.1

UKCEH and CEH Sections/Science Areas:

Biodiversity (Science Area 2017-)

ISSN:

2398-502X

Additional Information. Not used in RCUK Gateway to Research.:

Open Access paper - full text available via Official URL link.

Additional Keywords:

Earth BioGenome Project, invertebrate, whole-genome sequencing, collecting, preservation, prioritisation