A novel Lepidoptera bioassay analysed using a reduced GUTS model

Badder, Claire; Bart, Sylvain ORCID: https://orcid.org/0000-0002-0290-5546; Robinson, Alex; Hesketh, Helen ORCID: https://orcid.org/0000-0003-1794-7658; Kille, Peter; Spurgeon, David J. ORCID: https://orcid.org/0000-0003-3264-8760. 2023 A novel Lepidoptera bioassay analysed using a reduced GUTS model. Ecotoxicology and Environmental Safety, 251, 114504. 8, pp. https://doi.org/10.1016/j.ecoenv.2023.114504

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Official URL: http://dx.doi.org/10.1016/j.ecoenv.2023.114504

Abstract/Summary

Lepidopteran species can be both pests and also beneficial pollinators for agricultural crops. However, despite these important roles, the effects of pesticides on this diverse taxa are relatively understudied. To facilitate the assessment of pesticides and other chemical hazards on this taxa, we present a novel bioassay capable of testing chemical sensitivity to lepidopteran larvae through dietary exposure. We used Mamestra brassicae caterpillars as a model lepidopteran and tested their sensitivity for the organophosphate insecticide chlorpyrifos. We exposed larvae to an artificial diet spiked with chlorpyrifos and monitored survival over time, as well as weight change over a 96-hour exposure period. To test the repeatability and reliability of the developed bioassay, the experiment was repeated three times. The survival in time data collected enabled analysis with the General Unified Threshold of Survival (GUTS) model, recently recognized by EFSA as a ready-to-use tool for regulatory purposes. The GUTS modelling was used to derive a set of relevant toxicokinetic and toxicodynamic parameters relating to the larval response to exposure over time. We found that across the three repeats studies there was no more than a threefold difference in LC₅₀ values (13.1, 18.7 and 8.1 mg/Kg) at 48 h and fourfold difference at 96 h, highlighting the repeatability of the bioassay. We also highlighted the potential of the method to observe sub-lethal effects such as changes in weight. Finally, we discuss the applications of this new bioassay method to chemical risk assessments and its potential for use in other scenarios, such as mixture or pulsed exposure testing.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.ecoenv.2023.114504
UKCEH and CEH Sections/Science Areas:	Biodiversity (Science Area 2017-) Pollution (Science Area 2017-)
ISSN:	0147-6513
Additional Information. Not used in RCUK Gateway to Research.:	Open Access paper - full text available via Official URL link.
Additional Keywords:	cabbage moth, insecticide, survival test, toxicokinetics, toxicodynamics, effect modelling
NORA Subject Terms:	Ecology and Environment Zoology
Date made live:	02 Nov 2023 11:12 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/535341

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1016/j.ecoenv.2023.114504

UKCEH and CEH Sections/Science Areas:

Biodiversity (Science Area 2017-)
Pollution (Science Area 2017-)

ISSN:

0147-6513

Additional Information. Not used in RCUK Gateway to Research.:

Open Access paper - full text available via Official URL link.

Additional Keywords:

cabbage moth, insecticide, survival test, toxicokinetics, toxicodynamics, effect modelling