Gut and faecal bacterial community of the terrestrial isopod Porcellionides pruinosus: potential use for monitoring exposure scenarios

Oliveira, Jacinta M.M.; Henriques, Isabel; Read, Daniel S. ORCID: https://orcid.org/0000-0001-8546-5154; Gweon, Hyun S. ORCID: https://orcid.org/0000-0002-6218-6301; Morgado, Rui G.; Peixoto, Sara; Correia, António; Soares, Amadeu M.V.M.; Loureiro, Susana. 2021 Gut and faecal bacterial community of the terrestrial isopod Porcellionides pruinosus: potential use for monitoring exposure scenarios. Ecotoxicology, 30 (10). 2096-2108. https://doi.org/10.1007/s10646-021-02477-4

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Official URL: http://dx.doi.org/10.1007/s10646-021-02477-4

Abstract/Summary

This work aimed to characterize the gut and faeces bacterial communities (BC) of Porcellionides pruinosus using high-throughput sequencing. Isopods were collected from the field and kept in laboratory conditions similar to those normally applied in ecotoxicology tests. Faeces and purged guts of isopods (n = 3 × 30) were analysed by pyrosequencing the V3-V4 region of 16 S rRNA encoding gene. Results showed that gut and faecal BCs were dominated by Proteobacteria, particularly by an OTU (Operational Taxonomic Unit) affiliated to genus Coxiella. Diversity and richness values were statistically higher for faecal BC, mainly due to the occurrence of several low-abundance phylotypes. These results may reflect faecal carriage of bacterial groups that cannot settle in the gut. BCs of P. pruinosus comprised: (1) common members of the soil microbiota, (2) bacterial symbionts, (3) bacteria related to host metabolic/ecological features, and (4) bacterial etiological agents. Comparison of BC of this isopod species with the BC from other invertebrates revealed common bacterial groups across taxa. The baseline information provided by this work will assist the design and data interpretation of future ecotoxicological or biomonitoring assays where the analysis of P. pruinosus BC should be included as an additional indicator.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1007/s10646-021-02477-4
UKCEH and CEH Sections/Science Areas:	Soils and Land Use (Science Area 2017-) UKCEH Fellows
ISSN:	0963-9292
Additional Information. Not used in RCUK Gateway to Research.:	Publisher link (see Related URLs) provides a read-only full-text copy of the published paper.
Additional Keywords:	Porcellionides pruinosus, bacterial community, faeces, guts, pyrosequencing, ecotoxicological indicator
NORA Subject Terms:	Biology and Microbiology
Related URLs:	Publisher
Date made live:	15 Dec 2021 12:04 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/531577

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1007/s10646-021-02477-4

UKCEH and CEH Sections/Science Areas:

Soils and Land Use (Science Area 2017-)
UKCEH Fellows

ISSN:

0963-9292

Additional Information. Not used in RCUK Gateway to Research.:

Publisher link (see Related URLs) provides a read-only full-text copy of the published paper.

Additional Keywords:

Porcellionides pruinosus, bacterial community, faeces, guts, pyrosequencing, ecotoxicological indicator