First microsatellite markers for the European robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity

Gwiazdowska, Aleksandra; Karpińska, Oliwia; Kamionka-Kanclerska, Katarzyna; Rowiński, Patryk; Panagiotopoulou, Hanna; Pomorski, Jan J.; Broughton, Richard K.; da Silva, Luis F.P.; Rutkowski, Robert. 2021 First microsatellite markers for the European robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity. Scientific Reports, 11, 18962. 9, pp. https://doi.org/10.1038/s41598-021-98364-3

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Official URL: http://dx.doi.org/10.1038/s41598-021-98364-3

Abstract/Summary

The European Robin is a small passerine bird associated with woodlands of Eurasia and North Africa. Despite being relatively widespread and common, little is known of the species’ breeding biology and genetic diversity. We used Next Generation Sequencing (NGS) to develop and characterize microsatellite markers for the European Robin, designing three multiplex panels to amplify 14 microsatellite loci. The level of polymorphism and its value for assessing parentage and genetic structure was estimated based on 119 individuals, including seven full families and 69 unrelated individuals form Poland’s Białowieża Primaeval Forest and an additional location in Portugal. All markers appeared to be highly variable. Analysis at the family level confirmed a Mendelian manner of inheritance in the investigated loci. Genetic data also revealed evidence for extra-pair paternity in one family. The set of markers that we developed are proven to be valuable for analysis of the breeding biology and population genetics of the European Robin.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1038/s41598-021-98364-3
UKCEH and CEH Sections/Science Areas:	Biodiversity (Science Area 2017-)
ISSN:	2045-2322
Additional Information. Not used in RCUK Gateway to Research.:	Open Access paper - full text available via Official URL link.
Additional Keywords:	animal behaviour, ecological genetics, genetic markers, genetics, molecular ecology, population genetics, zoology
NORA Subject Terms:	Ecology and Environment
Date made live:	07 Oct 2021 15:46 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/531203

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1038/s41598-021-98364-3

UKCEH and CEH Sections/Science Areas:

Biodiversity (Science Area 2017-)

ISSN:

2045-2322

Additional Information. Not used in RCUK Gateway to Research.:

Open Access paper - full text available via Official URL link.

Additional Keywords:

animal behaviour, ecological genetics, genetic markers, genetics, molecular ecology, population genetics, zoology