Whole-cell Escherichia coli-based bio-sensor assay for dual zinc oxide nanoparticle toxicity mechanisms

Mcquillan, Jonathan S.; Shaw, Andrew M.. 2014 Whole-cell Escherichia coli-based bio-sensor assay for dual zinc oxide nanoparticle toxicity mechanisms. Biosensors and Bioelectronics, 51. 274-279. https://doi.org/10.1016/j.bios.2013.07.024

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Official URL: http://dx.doi.org/10.1016/j.bios.2013.07.024

Abstract/Summary

A whole-cell biosensor assay for dual ZnO nanoparticle toxicity mechanisms has been developed based on the transcriptional response of Escherichia coli to: (1) Zn2+ from ZnO nanoparticle dissolution with genes zntA (Zn2+ efflux) and znuABC (Zn2+ uptake); and (2) redox stress from ZnO nanoparticle photo-electron production under ultraviolet light with genes soxS and katG. Both processes occur in a dispersion of ZnO nanoparticles leading to toxicity. ZnO nanoparticle dissolution was measured independently by ICP-MS and photo-radical generation was confirmed by the stochiometric reduction of the redox dye, 2, 6-dichloroindolphenol (DCPIP). The whole-cell biosensor can detect both toxicity mechanisms and is a species-specific assay capable of discriminating between ZnO nanoparticles and the Zn2+ dissolution product.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.bios.2013.07.024
ISSN:	09565663
Date made live:	29 Oct 2019 13:27 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/525529

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1016/j.bios.2013.07.024

ISSN:

09565663

Date made live:

29 Oct 2019 13:27 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/525529