Genomic mutations after multigenerational exposure of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles

Wamucho, Anye; Unrine, Jason M.; Kieran, Troy J.; Glenn, Travis C.; Schultz, Carolin L.; Farman, Mark; Svendsen, Claus ORCID: https://orcid.org/0000-0001-7281-647X; Spurgeon, David J. ORCID: https://orcid.org/0000-0003-3264-8760; Tsyusko, Olga V.. 2019 Genomic mutations after multigenerational exposure of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles. Environmental Pollution, 254 (B), 113078. 10, pp. https://doi.org/10.1016/j.envpol.2019.113078

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Official URL: http://dx.doi.org/10.1016/j.envpol.2019.113078

Abstract/Summary

Our previous study showed heritable reproductive toxicity in the nematode Caenorhabditis elegans after multigenerational exposure to AgNO3 and silver nanoparticles (Ag-NPs). The aim of this study was to determine whether such inheritable effects are correlated with induced germline mutations in C. elegans. Individual C. elegans lineages were exposed for 10 generations to equitoxic concentrations at EC30 of AgNO3, Ag-NPs, and sulfidized Ag-NPs (sAg-NPs), a predominant environmentally transformed product of pristine Ag-NPs. The mutations were detected via whole genome DNA sequencing approach by comparing F0 and F10 generations. An increase in the total number of variants, though not statistically significant, was observed for all Ag treatments and the variants were mainly contributed by single nucleotide polymorphisms (SNPs). This potentially contributed towards reproductive as well as growth toxicity shown previously after ten generations of exposure in every Ag treatment. However, despite Ag-NPs and AgNO3 inducing stronger reproductive toxicity than sAg-NPs, exposure to sAg-NPs resulted in higher mutation accumulation with significant increase in the number of transversions. Thus our results suggest that other mechanisms of inheritance, such as epigenetics, may be at play in Ag-NP- and AgNO3-induced multigenerational and transgenerational reproductive toxicity.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.envpol.2019.113078
UKCEH and CEH Sections/Science Areas:	Pollution (Science Area 2017-)
ISSN:	0269-7491
Additional Keywords:	genotoxicity, germline, nanomaterials, nematode, variants
NORA Subject Terms:	Biology and Microbiology
Date made live:	09 Sep 2019 11:50 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/525018

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1016/j.envpol.2019.113078

UKCEH and CEH Sections/Science Areas:

Pollution (Science Area 2017-)

ISSN:

0269-7491

Additional Keywords:

genotoxicity, germline, nanomaterials, nematode, variants

NORA Subject Terms:

Biology and Microbiology