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Corrigendum to “A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters” [Water Res. 126, 101–110]

Walker, David I.; McQuillan, Jonathan; Taiwo, Michael; Parks, Rachel; Stenton, Craig A.; Morgan, Hywel; Mowlem, Matthew C.; Lees, David N.. 2019 Corrigendum to “A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters” [Water Res. 126, 101–110]. Water Research, 161. 652. https://doi.org/10.1016/j.watres.2019.06.070

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Abstract/Summary

It has been brought to our attention that there is an error in the Methods, section 2.6 “Real-time PCR”. The article as published states “Each reaction contained 12.5 μl of IQ SYBR Supermix con- taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 ml of each primer (final concentration 5 mM) and 1 ml of template DNA at 100 ng/μl; the final volume was 25 μl.”. However this should be “Each reaction contained 12.5 μl of IQ SYBR Supermix con- taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 μl of each primer (final concentration 0.4 μM) and 1 μl of template DNA at 100 ng/μl; the final volume was 25 μl.”

Item Type: Publication - Article
Digital Object Identifier (DOI): https://doi.org/10.1016/j.watres.2019.06.070
ISSN: 00431354
Date made live: 12 Aug 2019 09:50 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/524700

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