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A new widespread subclass of carbonic anhydrase in marine phytoplankton

Jensen, Erik L.; Clement, Romain; Kosta, Artemis; Maberly, Stephen C. ORCID: https://orcid.org/0000-0003-3541-5903; Gontero, Brigitte. 2019 A new widespread subclass of carbonic anhydrase in marine phytoplankton. ISME Journal, 13 (8). 2094-2106. https://doi.org/10.1038/s41396-019-0426-8

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Abstract/Summary

Most aquatic photoautotrophs depend on CO2-concentrating mechanisms (CCMs) to maintain productivity at ambient concentrations of CO2, and carbonic anhydrase (CA) plays a key role in these processes. Here we present different lines of evidence showing that the protein LCIP63, identified in the marine diatom Thalassiosira pseudonana, is a CA. However, sequence analysis showed that it has a low identity with any known CA and therefore belongs to a new subclass that we designate as iota-CA. Moreover, LCIP63 unusually prefers Mn2+ to Zn2+ as a cofactor, which is potentially of ecological relevance since Mn2+ is more abundant than Zn2+ in the ocean. LCIP63 is located in the chloroplast and only expressed at low concentrations of CO2. When overexpressed using biolistic transformation, the rate of photosynthesis at limiting concentrations of dissolved inorganic carbon increased, confirming its role in the CCM. LCIP63 homologs are present in the five other sequenced diatoms and in other algae, bacteria, and archaea. Thus LCIP63 is phylogenetically widespread but overlooked. Analysis of the Tara Oceans database confirmed this and showed that LCIP63 is widely distributed in marine environments and is therefore likely to play an important role in global biogeochemical carbon cycling.

Item Type: Publication - Article
Digital Object Identifier (DOI): https://doi.org/10.1038/s41396-019-0426-8
CEH Sections/Science Areas: Water Resources (Science Area 2017-)
ISSN: 1751-7362
Additional Information. Not used in RCUK Gateway to Research.: Open Access paper - full text available via Official URL link.
Additional Keywords: environmental chemistry, water microbiology
NORA Subject Terms: Marine Sciences
Biology and Microbiology
Date made live: 04 Jun 2019 11:08 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/523611

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