Increase of apoplastic ascorbate induced by ozone is insufficient to remove the negative effects in tobacco, soybean and poplar

Dai, Lulu; Feng, Zhaozhong; Pan, Xiaodong; Xu, Yansen; Li, Pin; Lefohn, Allen S.; Harmens, Harry; Kobayashi, Kazuhiko. 2019 Increase of apoplastic ascorbate induced by ozone is insufficient to remove the negative effects in tobacco, soybean and poplar. Environmental Pollution, 245. 380-388. https://doi.org/10.1016/j.envpol.2018.11.030

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Official URL: https://doi.org/10.1016/j.envpol.2018.11.030

Abstract/Summary

Apoplastic ascorbate (ASCapo) is an important contributor to the detoxification of ozone (O3). The objective of the study is to explore whether ASCapo is stimulated by elevated O3 concentrations. The detoxification of O3 by ASCapo was quantified in tobacco (Nicotiana L), soybean (Glycine max (L.) Merr.) and poplar (Populus L), which were exposed to charcoal-filtered air (CF) and elevated O3 treatments (E-O3). ASCapo in the three species were significantly increased by E-O3 compared with the values in the filtered treatment. For all three species, E-O3 significantly increased the malondialdehyde (MDA) content and decreased light-saturated rate of photosynthesis (Asat), suggesting that high O3 has induced injury/damage to plants. E-O3 significantly increased redox state in the apoplast (redox stateapo) for all species, whereas no effect on the apoplastic dehydroascorbate (DHAapo) was observed. In leaf tissues, E-O3 significantly enhanced reduced-ascorbate (ASC) and total ascorbate (ASC+DHA) in soybean and poplar, but significantly reduced these in tobacco, indicating different antioxidative capacity to the high O3 levels among the three species. Total antioxidant capacity in the apoplast (TACapo) was significantly increased by E-O3 in tobacco and poplar, but leaf tissue TAC was significantly enhanced only in tobacco. Leaf tissue superoxide anion (O2•-) in poplar and hydrogen peroxide (H2O2) in tobacco and soybean were significantly increased by E-O3. The diurnal variation of ASCapo, with maximum values occurring in the late morning with lower values experienced in the afternoon appeared to play an important role in the harmful effects of O3 on tobacco, soybean and poplar.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.envpol.2018.11.030
UKCEH and CEH Sections/Science Areas:	Soils and Land Use (Science Area 2017-)
ISSN:	0269-7491
Additional Keywords:	apoplast, ascorbate, detoxification, ozone, plants, reactive oxygen species, total antioxidant capacity
NORA Subject Terms:	Ecology and Environment Atmospheric Sciences Botany
Date made live:	14 Nov 2018 12:46 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/521503

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1016/j.envpol.2018.11.030

UKCEH and CEH Sections/Science Areas:

Soils and Land Use (Science Area 2017-)

ISSN:

0269-7491

Additional Keywords:

apoplast, ascorbate, detoxification, ozone, plants, reactive oxygen species, total antioxidant capacity

NORA Subject Terms:

Ecology and Environment
Atmospheric Sciences
Botany