Arbuscular mycorrhizal fungi and plant chemical defence: effects of colonisation on aboveground and belowground metabolomes

Hill, Elizabeth M.; Robinson, Lynne A.; Abdul-Sada, Ali; Vanbergen, Adam J. ORCID: https://orcid.org/0000-0001-8320-5535; Hodge, Angela; Hartley, Sue E.. 2018 Arbuscular mycorrhizal fungi and plant chemical defence: effects of colonisation on aboveground and belowground metabolomes. Journal of Chemical Ecology, 44 (2). 198-208. https://doi.org/10.1007/s10886-017-0921-1

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Official URL: http://dx.doi.org/10.1007/s10886-017-0921-1

Abstract/Summary

Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizophagus irregularis on the chemical defences, spanning above- and below-ground tissues, in its host-plant ragwort (Senecio jacobaea). We used a non-targeted metabolomics approach to profile, and where possible identify, compounds induced by AMF colonisation in both roots and shoots. Metabolomics analyses revealed that 33 compounds were significantly increased in the root tissue of AMF colonised plants, including seven blumenols, plant-derived compounds known to be associated with AMF colonisation. One of these was a novel structure conjugated with a malonyl-sugar and uronic acid moiety, hitherto an unreported combination. Such structural modifications of blumenols could be significant for their previously reported functional roles associated with the establishment and maintenance of AM colonisation. Pyrrolizidine alkaloids (PAs), key anti-herbivore defence compounds in ragwort, dominated the metabolomic profiles of root and shoot extracts. Analyses of the metabolomic profiles revealed an increase in four PAs in roots (but not shoots) of AMF colonised plants, with the potential to protect colonised plants from below-ground organisms.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1007/s10886-017-0921-1
UKCEH and CEH Sections/Science Areas:	Biodiversity (Science Area 2017-) Unaffiliated
ISSN:	0098-0331
Additional Information. Not used in RCUK Gateway to Research.:	Open Access paper - full text available via Official URL link.
Additional Keywords:	arbuscular mycorrhizal symbiosis, above-belowground interactions, phytobiome, microbiome, metabolomics, pyrrolizidine alkaloids, blumenols, Senecio jacobaea, Rhizophagus irregularis
NORA Subject Terms:	Ecology and Environment
Date made live:	21 Feb 2018 14:02 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/519362

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1007/s10886-017-0921-1

UKCEH and CEH Sections/Science Areas:

Biodiversity (Science Area 2017-)
Unaffiliated

ISSN:

0098-0331

Additional Information. Not used in RCUK Gateway to Research.:

Open Access paper - full text available via Official URL link.

Additional Keywords:

arbuscular mycorrhizal symbiosis, above-belowground interactions, phytobiome, microbiome, metabolomics, pyrrolizidine alkaloids, blumenols, Senecio jacobaea, Rhizophagus irregularis

NORA Subject Terms:

Ecology and Environment

Date made live:

21 Feb 2018 14:02 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/519362