Novel multi-isotope tracer approach to test ZnO nanoparticle and soluble Zn bioavailability in joint soil exposures

Laycock, Adam; Romero-Freire, Ana; Najorka, Jens; Svendsen, Claus ORCID: https://orcid.org/0000-0001-7281-647X; van Gestel, Cornelis A.M.; Rehkämper, Mark. 2017 Novel multi-isotope tracer approach to test ZnO nanoparticle and soluble Zn bioavailability in joint soil exposures. Environmental Science & Technology, 51 (21). 12756-12763. https://doi.org/10.1021/acs.est.7b02944

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Official URL: https://doi.org/10.1021/acs.est.7b02944

Abstract/Summary

Here we use two enriched stable isotopes, 68Znen and 64Znen (>99%), to prepare 68ZnO nanoparticles (NPs) and soluble 64ZnCl2. The standard LUFA 2.2 test soil was dosed with 68ZnO NPs and soluble 64ZnCl2 to 5 mg kg–1 each, plus between 0 and 95 mg kg–1 of soluble ZnCl2 with a natural isotope composition. After 0, 1, 3, 6, and 12 months of soil incubation, earthworms (Eisenia andrei) were introduced for 72 h exposures. Analyses of soils, pore waters, and earthworm tissues using multiple collector inductively coupled plasma mass spectrometry allowed the simultaneous measurement of the diagnostic 68Zn/66Zn, 64Zn/66Zn, and 68Zn/64Zn ratios, from which the three different isotopic forms of Zn were quantified. Eisenia andrei was able to regulate Zn body concentrations with no difference observed between the different total dosing concentrations. The accumulation of labeled Zn by the earthworms showed a direct relationship with the proportion of labeled to total Zn in the pore water, which increased with longer soil incubation times and decreasing soil pH. The 68Znen/64Znen ratios determined for earthworms (1.09 ± 0.04), soils (1.09 ± 0.02), and pore waters (1.08 ± 0.02) indicate indistinguishable environmental distribution and uptake of the Zn forms, most likely due to rapid dissolution of the ZnO NPs.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1021/acs.est.7b02944
UKCEH and CEH Sections/Science Areas:	Pollution (Science Area 2017-)
ISSN:	0013-936X
Additional Information. Not used in RCUK Gateway to Research.:	Open Access papaer - full text available via Official URL link.
NORA Subject Terms:	Ecology and Environment Agriculture and Soil Science
Date made live:	30 Nov 2017 11:08 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/518540

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1021/acs.est.7b02944

UKCEH and CEH Sections/Science Areas:

Pollution (Science Area 2017-)

ISSN:

0013-936X

Additional Information. Not used in RCUK Gateway to Research.:

Open Access papaer - full text available via Official URL link.

NORA Subject Terms:

Ecology and Environment
Agriculture and Soil Science