Extraction and characterization of collagen from Antarctic and Sub-Antarctic squid and its potential application in hybrid scaffolds for tissue engineering

Coelho, Rui C.G.; Marques, Ana L.P.; Oliveira, Sara M.; Diogo, Gabriela S.; Pirraco, Rogério P.; Moreira-Silva, Joana; Xavier, Jose C. ORCID: https://orcid.org/0000-0002-9621-6660; Reis, Rui L.; Silva, Tiago H.; Mano, João F.. 2017 Extraction and characterization of collagen from Antarctic and Sub-Antarctic squid and its potential application in hybrid scaffolds for tissue engineering. Materials Science and Engineering: C, 78. 787-795. 10.1016/j.msec.2017.04.122

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Official URL: https://doi.org/10.1016/j.msec.2017.04.122

Abstract/Summary

Collagen is the most abundant protein found in mammals and it exhibits a low immunogenicity, high biocompatibility and biodegradability when compared with others natural polymers. For this reason, it has been explored for the development of biologically instructive biomaterials with applications for tissue substitution and regeneration. Marine origin collagen has been pursued as an alternative to the more common bovine and porcine origins. This study focused on squid (Teuthoidea: Cephalopoda), particularly the Antarctic squid Kondakovia longimana and the Sub-Antarctic squid Illex argentinus as potential collagen sources. In this study, collagen has been isolated from the skins of the squids using acid-based and pepsin-based protocols, with the higher yield being obtained from I. argentinus in the presence of pepsin. The produced collagen has been characterized in terms of physicochemical properties, evidencing an amino acid profile similar to the one of calf collagen, but exhibiting a less preserved structure, with hydrolyzed portions and a lower melting temperature. Pepsin-soluble collagen isolated from I. argentinus was selected for further evaluation of biomedical potential, exploring its incorporation on poly-ε-caprolactone (PCL) 3D printed scaffolds for the development of hybrid scaffolds for tissue engineering, exhibiting hierarchical features

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.msec.2017.04.122
ISSN:	09284931
Additional Keywords:	squid collagen, Antarctic, three dimensional hybrid scaffolds, 3D printing, marine biomaterial, hierarchical scaffolds
NORA Subject Terms:	Medicine
Date made live:	01 Aug 2017 08:44 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/517443

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.msec.2017.04.122

ISSN:

09284931

Additional Keywords:

squid collagen, Antarctic, three dimensional hybrid scaffolds, 3D printing, marine biomaterial, hierarchical scaffolds

NORA Subject Terms:

Medicine

Date made live:

01 Aug 2017 08:44 +0 (UTC)