Incidence and spread of Haemophilus influenzae on an Antarctic base determined using the polymerase chain reaction

Hobson, R. P.; Williams, A.; Rawal, K.; Pennington, T. H.; Forbes, K. J.. 1995 Incidence and spread of Haemophilus influenzae on an Antarctic base determined using the polymerase chain reaction. Epidemiology and Infection, 114 (1). 93-103. 10.1017/S0950268800051943

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Abstract/Summary

A PCR-based method of detecting Haemophilus influenzae in cultures inoculated from throat swabs was evaluated using samples from groups of laboratory staff and medical students and then applied to samples originating from the closed human community of an Antarctic research station. Suitable PCR primers to an H. influenzae gene (ompP2) were used to amplify the gene from DNA preparations made from mixed growth on chocolate agar with added vancomycin. PCR product was reamplified and subjected to restriction endonuclease digestion to allow temporal and spatial mapping of strains over an 8-month period. Eleven different strains of H. influenzae were detected. One particular strain was detected in a third of the base members

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1017/S0950268800051943
Programmes:	BAS Programmes > Pre 2000 programme
ISSN:	0950-2688
NORA Subject Terms:	Medicine
Date made live:	20 Dec 2016 13:43 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/515605

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