Identification of a periplasmic dimethylsulphoxide reductase in Hyphomicrobium EG grown under chemolithoheterotrophic conditions with dimethylsulphoxide as carbon source

Hatton, Angela D.; Malin, Gill; McEwan, Alastair G.. 1994 Identification of a periplasmic dimethylsulphoxide reductase in Hyphomicrobium EG grown under chemolithoheterotrophic conditions with dimethylsulphoxide as carbon source. Archives of Microbiology, 162 (1-2). 148-150. https://doi.org/10.1007/BF00264389

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Official URL: http://dx.doi.org/10.1007/BF00264389

Abstract/Summary

Hyphomicrobium EG can grow with dimethylsulphoxide as sole carbon and energy source with oxygen as electron acceptor. In the present work we have found that the dimethylsulphoxide reductase of this bacterium could be assayed with dithionite-reduced methylviologen as reductant but not with NADH. Sub-cellular fractionation of Hyphomicrobium EG showed that the dimethylsulphoxide reductase was a periplasmic enzyme. An antibody to the dimethylsulphoxide reductase of Rhodobacter capsulatus cross-reacted with a polypeptide in the periplasmic fraction from Hyphomicrobium EG which had the same Mr as the dimethylsulphoxide reductase of Rhodobacter capsulatus. It is suggested that the reduction of dimethylsulphoxide in Hyphomicrobium involves respiratory electron transfer.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1007/BF00264389
ISSN:	0302-8933
Additional Keywords:	Hyphomicrobium; Dimethylsulphoxide reductase; Periplasmic enzymes; Chemolithoheterotrophic growth
Date made live:	05 Dec 2016 11:42 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/514534

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1007/BF00264389

ISSN:

0302-8933

Additional Keywords:

Hyphomicrobium; Dimethylsulphoxide reductase; Periplasmic enzymes; Chemolithoheterotrophic growth

Date made live:

05 Dec 2016 11:42 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/514534