Mutational bias of Turnip Yellow Mosaic Virus in the context of host anti-viral gene silencing

Ma, Jinmin; Pallett, Denise; Jiang, Hui; Hou, Yong; Wang, Hui. 2015 Mutational bias of Turnip Yellow Mosaic Virus in the context of host anti-viral gene silencing. Virology, 486. 2-6. https://doi.org/10.1016/j.virol.2015.08.024

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Official URL: http://dx.doi.org/10.1016/j.virol.2015.08.024

Abstract/Summary

Plant Dicer-like (DCL) enzymes exhibit a GC-preference during anti-viral post-transcriptional gene silencing (PTGS), delivering an evolutionary selection pressure resulting in plant viruses with GC-poor genomes. However, some viruses, e.g. Turnip Yellow Mosaic Virus (TYMV, genus Tymovirus) have GC-rich genomes, raising the question as to whether or not DCL derived selection pressure affects these viruses. In this study we analyzed the virus-derived small interfering RNAs from TYMV-infected leaves of Brassica juncea showed that the TYMV population accumulated a mutational bias with AU replacing GC (GC–AU), demonstrating PTGS pressure. Interestingly, at the highly polymorphic sites the GC–AU bias was no longer observed. This suggests the presence of an unknown mechanism preventing mutational drift of the viral population and maintaining viral genome stability, despite the host PTGS pressure.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.virol.2015.08.024
UKCEH and CEH Sections/Science Areas:	UKCEH Fellows Reynard
ISSN:	0042-6822
Additional Keywords:	anti-viral post-transcriptional gene silencing, Turnip Yellow Mosaic Virus, Brassica juncea, virus population, mutational bias
NORA Subject Terms:	Ecology and Environment Agriculture and Soil Science Biology and Microbiology
Date made live:	16 Sep 2015 09:16 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/511774

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1016/j.virol.2015.08.024

UKCEH and CEH Sections/Science Areas:

UKCEH Fellows
Reynard

ISSN:

0042-6822

Additional Keywords:

anti-viral post-transcriptional gene silencing, Turnip Yellow Mosaic Virus, Brassica juncea, virus population, mutational bias

NORA Subject Terms:

Ecology and Environment
Agriculture and Soil Science
Biology and Microbiology