Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically-driven continuous fluid samplers

Robidart, Julie; Callister, Stephen J.; Song, Pengfei; Nicora, Carrie D.; Wheat, Charles G.; Girguis, Peter R.

Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically-driven continuous fluid samplers

Robidart, Julie ORCID: https://orcid.org/0000-0001-9805-3570; Callister, Stephen J.; Song, Pengfei; Nicora, Carrie D.; Wheat, Charles G.; Girguis, Peter R.. 2013 Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically-driven continuous fluid samplers. Environmental Science & Technology, 47 (9). 4399-4407. 10.1021/es3037302

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Official URL: http://dx.doi.org/10.1021/es3037302

Abstract/Summary

Microbes play a key role in mediating aquatic biogeochemical cycles. However, our understanding of the relationships between microbial phylogenetic/physiological diversity and habitat physicochemical characteristics is restrained by our limited capacity to concurrently collect microbial and geochemical samples at appropriate spatial and temporal scales. Accordingly, we have developed a low-cost, continuous fluid sampling system (the Biological OsmoSampling System, or BOSS) to address this limitation. The BOSS does not use electricity, can be deployed in harsh/remote environments, and collects/preserves samples with daily resolution for >1 year. Here, we present data on the efficacy of DNA and protein preservation during a 1.5 year laboratory study as well as the results of two field deployments at deep-sea hydrothermal vents, wherein we examined changes in microbial diversity, protein expression, and geochemistry over time. Our data reveal marked changes in microbial composition co-occurring with changes in hydrothermal fluid composition as well as the temporal dynamics of an enigmatic sulfide-oxidizing symbiont in its free-living state. We also present the first data on in situ protein preservation and expression dynamics highlighting the BOSS’s potential utility in meta-proteomic studies. These data illustrate the value of using BOSS to study relationships among microbial and geochemical phenomena and environmental conditions.

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1021/es3037302

ISSN:

0013-936X

Date made live:

02 Feb 2015 15:23 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/509555