A new medium for Caenorhabditis elegans toxicology and nanotoxicology studies designed to better reflect natural soil solution conditions

Tyne, William; Lofts, Stephen ORCID: https://orcid.org/0000-0002-3627-851X; Spurgeon, David J. ORCID: https://orcid.org/0000-0003-3264-8760; Jurkschat, Kerstin; Svendsen, Claus ORCID: https://orcid.org/0000-0001-7281-647X. 2013 A new medium for Caenorhabditis elegans toxicology and nanotoxicology studies designed to better reflect natural soil solution conditions. Environmental Toxicology and Chemistry, 32 (8). 1711-1717. https://doi.org/10.1002/Etc.2247

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/etc.224...

Abstract/Summary

A new toxicity test medium for Caenorhabditis elegans is presented. The test solution is designed to provide a better representation of natural soil pore water conditions than currently available test media. The medium has a composition that can readily be modified to allow for studies of the influences of a range of environmentally relevant parameters on nematode biology and toxicology. Tests conducted in the new medium confirmed that nematodes' reproduction was possible at a range of solution pH levels, offering the potential to conduct toxicity studies under a variety of conditions. A test to establish silver nanoparticle and dissolved silver nitrate toxicity, a study type not feasible in M9 or agar media due to precipitation and nanoparticle agglomeration, indicated lower silver nanoparticle (median effective concentration [EC50] of 6.5 mg Ag/L) than silver nitrate (EC50 0.28 mg Ag/L) toxicity. Characterization identified stable nanoparticle behavior in the new test medium.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1002/Etc.2247
Programmes:	CEH Topics & Objectives 2009 - 2012 > Biogeochemistry > BGC Topic 3 - Managing Threats to Environment and Health
UKCEH and CEH Sections/Science Areas:	Acreman Shore
ISSN:	0730-7268
Additional Keywords:	nanoparticle, silver, toxicity test, ionic strength, fulvic acid, pH
NORA Subject Terms:	Ecology and Environment
Date made live:	27 Sep 2013 15:03 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/503275

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1002/Etc.2247

Programmes:

CEH Topics & Objectives 2009 - 2012 > Biogeochemistry > BGC Topic 3 - Managing Threats to Environment and Health

UKCEH and CEH Sections/Science Areas:

Acreman
Shore

ISSN:

0730-7268

Additional Keywords:

nanoparticle, silver, toxicity test, ionic strength, fulvic acid, pH