The HSP70 heat shock response in the Antarctic fish Harpagifer antarcticus.

Clark, Melody S. ORCID: https://orcid.org/0000-0002-3442-3824; Fraser, Keiron P.P. ORCID: https://orcid.org/0000-0001-5491-8376; Burns, Gavin; Peck, Lloyd S. ORCID: https://orcid.org/0000-0003-3479-6791. 2008 The HSP70 heat shock response in the Antarctic fish Harpagifer antarcticus. Polar Biology, 31 (2). 171-180. https://doi.org/10.1007/s00300-007-0344-5

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Official URL: http://dx.doi.org/10.1007/s00300-007-0344-5

Abstract/Summary

Members of the HSP70 gene family comprising the constitutive (HSC70) and inducible (HSP70) genes, plus GRP78 (Glucose-regulated protein 78 kDa) were surveyed for expression levels via Q-PCR after both an acute 2-h heat shock experiment and a time course assay in the Antarctic plunderfish Harpagifer antarcticus. In general, down regulation of all genes was observed during the course of the heat shock experiments. This thermally induced down regulation was particularly acute for the GRP78 gene, which at one time point was more than 100-fold down regulated. These results demonstrate the loss of the heat shock response in H. antarcticus, a basal member of the Notothenioidei. This finding is discussed with reference to the survival of Notothenioids during observed ocean warming and also the reorganisation of cellular protein mechanisms of species living in extreme environments.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1007/s00300-007-0344-5
Programmes:	BAS Programmes > Global Science in the Antarctic Context (2005-2009) > Biodiversity, Functions, Limits and Adaptation from Molecules to Ecosystems
ISSN:	0722-4060
Additional Keywords:	Antarctic, Climate change, Stenothermal, Heat shock protein, Marine
NORA Subject Terms:	Biology and Microbiology Zoology Ecology and Environment
Date made live:	29 Aug 2008 13:39 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/3961

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1007/s00300-007-0344-5

Programmes:

BAS Programmes > Global Science in the Antarctic Context (2005-2009) > Biodiversity, Functions, Limits and Adaptation from Molecules to Ecosystems

ISSN:

0722-4060

Additional Keywords:

Antarctic, Climate change, Stenothermal, Heat shock protein, Marine

NORA Subject Terms:

Biology and Microbiology
Zoology
Ecology and Environment