Thermodynamic and kinetic controls on cotransport of Pantoea agglomerans cells and Zn through clean and iron oxide coated sand columns

Kapetas, Leon; Ngwenya, Bryne T.; MacDonald, Alan ORCID: https://orcid.org/0000-0001-6636-1499; Elphick, Stephen C.. 2012 Thermodynamic and kinetic controls on cotransport of Pantoea agglomerans cells and Zn through clean and iron oxide coated sand columns. Environmental Science and Technology, 46 (24). 13193-13201. https://doi.org/10.1021/es302801a

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Abstract/Summary

Recent observations that subsurface bacteria quickly adsorb metal contaminants raise concerns that they may enhance metal transport, given the high mobility of bacteria themselves. However, metal adsorption to bacteria is also reversible, suggesting that mobility within porous medium will depend on the interplay between adsorption desorption kinetics and thermodynamic driving forces for adsorption. Till now there has been no systematic investigation of these important interactions. This study investigates the thermodynamic and kinetic controls of co-transport of Pantoea agglomerans cells and Zn in quartz and iron-oxide coated sand (IOCS) packed columns. Batch kinetic studies show that significant Zn sorption on IOCS takes place within two hours. Adsorption onto P. agglomerans surfaces reaches equilibrium within 30 minutes. Experiments in flow through quartz sand systems demonstrate that bacteria have negligible effect on zinc mobility, regardless of ionic strength and pH conditions. Zinc transport exhibits significant retardation in IOCS columns at high pH in the absence of cells. Yet, when mobile bacteria (non attached) are passed through simultaneously with zinc, no facilitated transport is observed. Adsorption onto cells becomes significant and plays a role in mobile metal speciation only once the IOCS is saturated with zinc. This suggests that IOCS exhibits stronger affinity for Zn than cell surfaces. However, when bacteria and Zn are pre-associated on entering the column, zinc transport is initially facilitated. Subsequently, zinc partly desorbs from the cells and redistributes onto the IOCS as a result of the higher thermodynamic affinity for IOCS.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1021/es302801a
Programmes:	BGS Programmes 2010 > Groundwater Science
Additional Keywords:	Thermodynamic Kinetic Cotransport Pantoea Agglomerans Cells Zn Iron Oxide
NORA Subject Terms:	Hydrology
Date made live:	04 Feb 2013 16:24 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/21407

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