Evidence for phenotypic plasticity among multihost Campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and Raman spectroscopy

Read, Daniel S. ORCID: https://orcid.org/0000-0001-8546-5154; Woodcock, Dan J.; Strachan, Norval J.C.; Forbes, Kenneth J.; Colles, Frances M.; Maiden, Martin C.J.; Clifton-Hadley, Felicity; Ridley, Anne; Vidal, Ana; Rodgers, John; Whiteley, Andrew S.; Sheppard, Samuel K.. 2013 Evidence for phenotypic plasticity among multihost Campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and Raman spectroscopy. Applied and Environmental Microbiology, 79 (3). 965-973. https://doi.org/10.1128/AEM.02521-12

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Abstract/Summary

Closely related bacterial isolates can display divergent phenotypes. This can limit the usefulness of phylogenetic studies for understanding bacterial ecology and evolution. Here we compare phenotyping, based on Raman spectrometric analysis of cellular composition, and phylogenetic classification by Ribosomal Multilocus Sequence Typing (rMLST) in 108 isolates of the zoonotic pathogens Campylobacter jejuni and C. coli. Automatic Relevance Determination (ARD) was used to identify informative peaks in the Raman spectra that could be used to distinguish strains in taxonomic and host source groups (species, clade, clonal complex, isolate source/host). Phenotypic characterization based on Raman spectra showed a degree of agreement with genotypic classification using rMLST, with segregation accuracy between species (83.95 %), clade (in C. coli, 98.41 %) and to some extent clonal complex (73.8 % C. jejuni ST-21 and ST-45 complexes) being achieved. This confirmed the utility of Raman spectroscopy for lineage classification and the correlation between genotypic and phenotypic classification. In parallel analysis, relatively distantly related isolates (different clonal complexes) were assigned the correct host origin irrespective of the clonal origin (74.1 - 97.0% accuracy) based upon different Raman peaks. This suggests that the phenotypic characteristics, from which the phenotypic signal is derived, are not fixed by clonal descent but are influenced by the host environment and may change as strains move between hosts.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1128/AEM.02521-12
Programmes:	CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 1 - Observations, Patterns, and Predictions for Biodiversity
UKCEH and CEH Sections/Science Areas:	Hails
ISSN:	0099-2240
Additional Information. Not used in RCUK Gateway to Research.:	Freely available on the publisher's website - click on Official URL link to access full text
NORA Subject Terms:	Biology and Microbiology
Date made live:	20 Dec 2012 15:46 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/20876

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