Metabolic profile biomarkers of metal contamination in a sentinel terrestrial species are applicable across multiple sites

Bundy, Jacob G.; Keun, Hector C.; Sidhu, Jasmin K.; Spurgeon, David J. ORCID: https://orcid.org/0000-0003-3264-8760; Svendsen, Claus ORCID: https://orcid.org/0000-0001-7281-647X; Kille, Peter; Morgan, A. John. 2007 Metabolic profile biomarkers of metal contamination in a sentinel terrestrial species are applicable across multiple sites. Environmental Science & Technology, 41 (12). 4458-4464. https://doi.org/10.1021/es0700303

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Abstract/Summary

In this study, we addressed the question of whether an omic approach could genuinely be useful for biomarker profile analysis across different field sites with different physicochemical characteristics. We collected earthworms (Lumbricus rubellus) from seven sites with very different levels of metal contamination and prevailing soil type and analyzed tissue extracts by 1H nuclear magnetic resonance spectroscopy. Pattern recognition analysis of the data showed that both site- and contaminant-specific effects on the metabolic profiles could be discerned. Zinc was identified as the probable major contaminant causing a metabolic change in the earthworms. Individual sites could be resolved on the basis of NMR spectral profiles by principal component analysis; these site differences may also have been caused by additional abiotic factors such as soil pH. Despite an inevitable degree of confounding between site and contaminant concentrations, it was possible to identify metabolites which were correlated with zinc across all different sites. This study therefore acts as a proof of principle for the use of NMR-based metabolic profiling as a diagnostic tool for ecotoxicological research in polluted field soils.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1021/es0700303
Programmes:	CEH Programmes pre-2009 publications > Biogeochemistry > SE01B Sustainable Monitoring, Risk Assessment and Management of Chemicals
UKCEH and CEH Sections/Science Areas:	Hails
ISSN:	0013-936X
NORA Subject Terms:	Agriculture and Soil Science Ecology and Environment
Date made live:	15 Jan 2008 12:48 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/2053

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1021/es0700303

Programmes:

CEH Programmes pre-2009 publications > Biogeochemistry > SE01B Sustainable Monitoring, Risk Assessment and Management of Chemicals

UKCEH and CEH Sections/Science Areas:

Hails

ISSN:

0013-936X

NORA Subject Terms:

Agriculture and Soil Science
Ecology and Environment