Identity and function of single microbial cells within a community by Raman microspectroscopy and related single-cell techniques

Read, Daniel S. ORCID: https://orcid.org/0000-0001-8546-5154; Whiteley, Andrew S.. 2011 Identity and function of single microbial cells within a community by Raman microspectroscopy and related single-cell techniques. In: Sen, Keya; Ashbolt, Nicholas J., (eds.) Environmental Microbiology: Current Technology and Water Applications. Horizon Press, 163-178.

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Abstract/Summary

Linking both identity and function within micro- bial communities has long been seen as essential for understanding the role that bacteria play in the environment. Techniques based on the study of single microbial cells offer a unique approach that provides information about heterogeneity within populations, and the role of spatial organisation within the environment. This chapter details some of the prominent single-cell techniques cur- rently in use for the study of microbial ecology, with a particular focus on Raman spectroscopy. A general overview of this technique is provided, with examples of its applicability for studying different features of microbial systems. Special at- tention is given to the use of Raman spectroscopy in combination with fluorescence in situ hybridi- sation (FISH) and stable isotope probing (SIP), which together can be utilized to gain an inSight into the identity and function of Single bacterial cells in situ.

Item Type:	Publication - Book Section
Programmes:	CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 1 - Observations, Patterns, and Predictions for Biodiversity
UKCEH and CEH Sections/Science Areas:	Hails
ISBN:	9781904455707
NORA Subject Terms:	Biology and Microbiology
Date made live:	30 Jan 2012 14:51 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/16557

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