Decay of cultivated apricot wood (Prunus armeniaca) by the ascomycete Hypocrea sulphurea using solid state 13CNMR and off-line TMAH thermochemolysis with GC-MS

Vane, Christopher H. ORCID: https://orcid.org/0000-0002-8150-3640; Drage, Trevor C.; Snape, Colin E.; Stephenson, Michael H.; Foster, Clinton. 2005 Decay of cultivated apricot wood (Prunus armeniaca) by the ascomycete Hypocrea sulphurea using solid state 13CNMR and off-line TMAH thermochemolysis with GC-MS. International Biodeterioration Biodegradation, 55 (3). 175-185. 10.1016/j.ibiod.2004.11.004

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Abstract/Summary

Chemical changes of polysaccharides and lignin in Prunus armeniaca decayed by the ascomycete fungus Hypocrea sulphurea were investigated. Solid-state 13C NMR spectra showed that polysaccharides were the main components of fresh and decayed wood. Decomposition of cellulose and hemicellulose by the fungus was minimal although a slight preference for crystalline as compared with amorphous cellulose was recognised. Comparison of the signal intensity of the resonance at 145 ppm in fresh and decomposed wood suggested that the fungus had decomposed tannin constituents. Thermochemolysis with tetramethylammonium hydroxide (TMAH) and product identification by gas chromatography–mass spectrometry revealed that the ratio of syringyl-type (S) units to guaiacyl-type (G) units decreased from 1.8 to 1.1 following fungal attack. Increases in both guaiacyl and syringyl acid-aldehyde ratios (Ad/Al)G, (Ad/Al)S together with an increase from 0.82 to 3.54 in the ratio of methyl 3,4-dimethoxybenzoate to the sum of 1-(3,4-dimethoxyphenyl)-1,2,3-trimethoxypropane (threo and erythro-isomers) ΓG from the decayed wood confirmed oxidative Cα–Cβ cleavage for the mechanism of lignin decay by this ascomycete.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.ibiod.2004.11.004
Programmes:	BGS Programmes > Other
ISSN:	0964-8305
Date made live:	25 Nov 2011 16:25 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/15981

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.ibiod.2004.11.004

Programmes:

BGS Programmes > Other

ISSN:

0964-8305

Date made live:

25 Nov 2011 16:25 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/15981