Field surveys reveal the presence of anti-androgens in an effluent-receiving river using stickleback-specific biomarkers

Katsiadaki, Ioanna; Sanders, Matthew B.; Henrys, Peter A. ORCID: https://orcid.org/0000-0003-4758-1482; Scott, Alexander P.; Matthiessen, Peter; Pottinger, Tom G.. 2012 Field surveys reveal the presence of anti-androgens in an effluent-receiving river using stickleback-specific biomarkers. Aquatic Toxicology, 122-123. 75-85. https://doi.org/10.1016/j.aquatox.2012.05.006

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Official URL: http://dx.doi.org/10.1016/j.aquatox.2012.05.006

Abstract/Summary

This study was designed to assess whether the removal of endocrine disrupting chemicals (EDCs) and other substances from a Waste Water Treatment Works (WWTW) effluent (receiving water: R. Ray, Swindon, UK) by granular activated carbon (GAC) affected biomarkers of exposure to EDCs [vitellogenin (VTG) and spiggin] in male and female three-spined sticklebacks in the receiving water. A nearby river (R. Ock), with a negligible effluent loading, was used as a control. On each river fish were sampled from four sites on five occasions both before and after remediation of the WWTW effluent. The results show for the first time in a UK field study a clear seasonality of blood VTG concentrations in wild male fish, following closely the VTG profile in female fish from both rivers. VTG levels in male fish from the R. Ray were significantly reduced after the GAC installation. However, VTG levels in males from the control sites also varied significantly across the same period, reducing the significance of this finding. A laboratory exposure to oestradiol (using site-specific lower and upper levels of oestrogenic activity) failed to elevate VTG concentrations in male sticklebacks suggesting that concentrations in the effluent, even prior to remediation, may not have exceeded a critical sensitivity threshold. Most importantly, a significant increase in female kidney spiggin content (a highly specific biomarker of xeno-androgen exposure) occurred in fish in the R. Ray after the GAC installation to levels comparable with those in fish from the control river. The significance of this finding is strengthened by the fact that during the pre-remediation period in the R. Ray, female spiggin levels increased with increasing distance from the WWTW. Our results provide the first in vivo evidence of the presence of anti-androgens in a U.K. WWTW effluent. To our knowledge this is the first U.K.-based comprehensive field study on the effects of a WWTW upgrade on biomarkers of EDC exposure using a sentinel fish species and our findings confirm the value of the stickleback as a model species for studying EDCs both in the laboratory and in the wild.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.aquatox.2012.05.006
Programmes:	CEH Topics & Objectives 2009 - 2012 > Water > WA Topic 2 - Ecohydrological Processes > WA - 2.3 - Assess the responses of river, lake and wetland ecosystems to ecohydrological drivers
UKCEH and CEH Sections/Science Areas:	UKCEH Fellows Parr
ISSN:	0166-445X
Additional Information. Not used in RCUK Gateway to Research.:	NOTICE: this is the author’s version of a work that was accepted for publication in Aquatic Toxicology, 46 (5). 1364-1371. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquatic Toxicology, 122-123. 75-85. 10.1016/j.aquatox.2012.05.006 www.elsevier.com/
Additional Keywords:	endocrine disruption, sewage effluent, vitellogenin, spiggin, three-spined stickleback, anti-androgens, wild fish survey
NORA Subject Terms:	Biology and Microbiology Zoology Ecology and Environment
Date made live:	29 May 2012 11:18 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/15122

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1016/j.aquatox.2012.05.006

Programmes:

CEH Topics & Objectives 2009 - 2012 > Water > WA Topic 2 - Ecohydrological Processes > WA - 2.3 - Assess the responses of river, lake and wetland ecosystems to ecohydrological drivers

UKCEH and CEH Sections/Science Areas:

UKCEH Fellows
Parr

ISSN:

0166-445X

Additional Information. Not used in RCUK Gateway to Research.:

NOTICE: this is the author’s version of a work that was accepted for publication in Aquatic Toxicology, 46 (5). 1364-1371. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquatic Toxicology, 122-123. 75-85. 10.1016/j.aquatox.2012.05.006 www.elsevier.com/

Additional Keywords:

endocrine disruption, sewage effluent, vitellogenin, spiggin, three-spined stickleback, anti-androgens, wild fish survey

NORA Subject Terms:

Biology and Microbiology
Zoology
Ecology and Environment

Date made live:

29 May 2012 11:18 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/15122