Functional and compositional comparison of two activated sludge communities remediating coking effluent

Manefield, Mike; Griffiths, Robert I. ORCID: https://orcid.org/0000-0002-3341-4547; Leigh, Mary Beth; Fisher, Ray; Whiteley, Andrew S.. 2005 Functional and compositional comparison of two activated sludge communities remediating coking effluent. Environmental Microbiology, 7 (5). 715-722. https://doi.org/10.1111/j.1462-2920.2004.00746.x

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1462-...

Abstract/Summary

The success of engineered microbiological systems is evident in the global application of activated sludge communities to remediate coking effluent. However, there is a lack of understanding of the microbiology underlying treatment efficiency and stability. In this study, two functionally distinct activated sludge pools, treating the same effluent and operating under the same conditions, were examined to establish a relationship between overall diversity and/or functional diversity with respect to process stability. Molecular profiling, sequencing and RNA-based stable isotope probing were used to examine the bacterial diversity, general composition and functional composition of the most abundant members of the two communities. The inferior process stability in one of the pools could not be explained by reduced total bacterial diversity or evenness. RNA-based stable isotope probing revealed that both pools harboured an abundant phenol-degrading Acidovorax species, and that the pool of inferior stability accommodated an additional closely related phenol-degrading Acidovorax species at high abundance. These results are discussed in the context of deterministic and stochastic models of microbial community assembly

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1111/j.1462-2920.2004.00746.x
Programmes:	CEH Programmes pre-2009 publications > Biodiversity
UKCEH and CEH Sections/Science Areas:	_ Molecular Microbial Ecology
ISSN:	1462-2912
NORA Subject Terms:	Biology and Microbiology
Date made live:	14 Dec 2011 09:41 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/13814

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1111/j.1462-2920.2004.00746.x

Programmes:

CEH Programmes pre-2009 publications > Biodiversity

UKCEH and CEH Sections/Science Areas:

_ Molecular Microbial Ecology

ISSN:

1462-2912

NORA Subject Terms:

Biology and Microbiology