RNA stable isotope probing, a novel means of linking microbial community function to Phylogeny

Manefield, Mike; Whiteley, Andrew S.; Griffiths, Robert I. ORCID: https://orcid.org/0000-0002-3341-4547; Bailey, Mark J.. 2002 RNA stable isotope probing, a novel means of linking microbial community function to Phylogeny. Applied and Environmental Microbiology, 68 (11). 5367-5373. https://doi.org/10.1128/AEM.68.11.5367-5373.2002

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Abstract/Summary

Identifying microorganisms responsible for recognized environmental processes remains a great challenge in contemporary microbial ecology. Only in the last few years have methodological innovations provided access to the relationship between the function of a microbial community and the phylogeny of the organisms accountable for it. In this study stable-isotope-labeled [13C]phenol was fed into a phenol-degrading community from an aerobic industrial bioreactor, and the 13C-labeled RNA produced was used to identify the bacteria responsible for the process. Stable-isotope-labeled RNA was analyzed by equilibrium density centrifugation in concert with reverse transcription-PCR and denaturing gradient gel electrophoresis. In contradiction with findings from conventional methodologies, this unique approach revealed that phenol degradation in the microbial community under investigation is dominated by a member of the Thauera genus. Our results suggest that this organism is important for the function of this bioreactor.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1128/AEM.68.11.5367-5373.2002
Programmes:	CEH Programmes pre-2009 publications > Other
UKCEH and CEH Sections/Science Areas:	_ Molecular Microbial Ecology
ISSN:	0099-2240
NORA Subject Terms:	Biology and Microbiology
Date made live:	26 Apr 2012 14:20 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/13813

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