Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition

Griffiths, Robert I. ORCID: https://orcid.org/0000-0002-3341-4547; Whiteley, Andrew S.; O'Donnell, Anthony G.; Bailey, Mark J.. 2000 Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition. Applied and Environmental Microbiology, 66 (12). 5488-5491.

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Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92488/

Abstract/Summary

A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription- PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.

Item Type:	Publication - Article
Programmes:	CEH Programmes pre-2009 publications > Biodiversity > BD03 The Genetic Basis of Ecological Function
UKCEH and CEH Sections/Science Areas:	_ Pre-2000 sections
ISSN:	0099-2240
NORA Subject Terms:	Biology and Microbiology
Date made live:	23 Aug 2011 08:34 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/13809

Item Type:

Publication - Article

Programmes:

CEH Programmes pre-2009 publications > Biodiversity > BD03 The Genetic Basis of Ecological Function

UKCEH and CEH Sections/Science Areas:

_ Pre-2000 sections

ISSN:

0099-2240

NORA Subject Terms:

Biology and Microbiology

Date made live:

23 Aug 2011 08:34 +0 (UTC)