Analysis of stress-induced hepatic gene expression in rainbow trout (Oncorhynchus mykiss) selected for high- and low-responsiveness to stress

Pemmasani, Jhansi K.; Pottinger, Tom G.; Cairns, Michael T.. 2011 Analysis of stress-induced hepatic gene expression in rainbow trout (Oncorhynchus mykiss) selected for high- and low-responsiveness to stress. Comparative Biochemistry and Physiology. Part D, Genomics and Proteomics, 6 (4). 406-419. 10.1016/j.cbd.2011.09.001

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Abstract/Summary

The production and welfare of intensively reared fish would be improved by reducing stress responsiveness. One approach to achieving this goal is selective breeding utilizing stress-responsive genes as direct genetic markers of the desirable trait. As a first step in this process, microarray analysis has been carried out on liver tissues of rainbow trout selectively bred for high (HR) or low (LR) responsiveness to a stressor. Microarray hybridizations provided gene expression profiles for pooled samples of fish confined for 6 h, 24 h and 168 h and for individual fish (168 h only). 161 genes were shown to be differentially regulated in HR and LR fish during confinement exposure and eight of these gene expression profiles were validated by quantitative PCR. Genes of particular interest included intelectin-2 precursor which showed greater than 100-fold higher expression in HR fish compared to LR fish irrespective of whether the fish were confined or not; interferon inducible transmembrane protein 3 which was differentially stress-induced between the two lines; and hepatic pro-opiomelanocortin B (POMC B) which was upregulated during stress in HR fish but downregulated in LR fish. All these offer potential as direct markers of low stress responsiveness in a marker-assisted selection scheme.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.cbd.2011.09.001
Programmes:	CEH Topics & Objectives 2009 - 2012 > Water
UKCEH and CEH Sections/Science Areas:	Parr
ISSN:	1744-117X
Additional Keywords:	fish, stress, aquaculture, selective breeding, time course, liver, microarray analysis, quantitative PCR, cortisol, genetic markers
NORA Subject Terms:	Biology and Microbiology Zoology
Date made live:	08 Nov 2011 14:38 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/12496

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.cbd.2011.09.001

Programmes:

CEH Topics & Objectives 2009 - 2012 > Water

UKCEH and CEH Sections/Science Areas:

Parr

ISSN:

1744-117X

Additional Keywords:

fish, stress, aquaculture, selective breeding, time course, liver, microarray analysis, quantitative PCR, cortisol, genetic markers