Metabolomic fingerprint of cryo-stress in a freeze tolerant insect

Hawes, T.C.; Hines, A.; Viant, M.R; Bale, J.S.; Worland, M. Roger; Convey, Peter ORCID: https://orcid.org/0000-0001-8497-9903. 2008 Metabolomic fingerprint of cryo-stress in a freeze tolerant insect. CryoLetters, 29 (6). 505-515.

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Abstract/Summary

This study employed H-1 NMR spectroscopy to assay the metabolome of the high Arctic freeze-tolerant dipteran larvae, Heleomyza borealis, after recovery from exposure to a range of sub-zero temperature treatments. Our data demonstrate the resilience of freeze tolerance in individuals of this permanently freeze-tolerant species that were acclimated to summer temperatures (5 degrees C): recovery of homeostasis after 48h was not significantly disturbed by 2h exposures to -3, -12, or -20 degrees C. Evidence of homeostatic perturbation to cryo-stress - both in terms of changes in specific metabolite concentrations as well as systemic changes in metabolism determined using multivariate pattern recognition techniques - was expressed almost entirely at a temperature coincident with the significant onset of mortality (-25 degrees C) and considerably below the minimum winter temperatures of its over-wintering habitat (c.-12 degrees C).

Item Type:	Publication - Article
Programmes:	BAS Programmes > Global Science in the Antarctic Context (2005-2009) > Biodiversity, Functions, Limits and Adaptation from Molecules to Ecosystems
ISSN:	0143-2044
Additional Keywords:	freeze tolerance; NMR; metabolomics; Heleomyza borealis; homeostasis; proline
NORA Subject Terms:	Biology and Microbiology Zoology Ecology and Environment
Date made live:	13 Jan 2011 14:37 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/11499

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