Raman FISH
Read, D.S. ORCID: https://orcid.org/0000-0001-8546-5154; Huang, W.E.; Whiteley, A.S.. 2009 Raman FISH. In: Timmis, Kenneth. N.; McGenity, Terry; Meer, Jan Roelof van der; Lorenzo, Victor de, (eds.) Handbook of Hydrocarbon and Lipid Microbiology. Springer, 4027-4038.
Full text not available from this repository.Abstract/Summary
Linking both identity and function within natural microbial communities has long been seen as one of the ultimate goals for microbial ecologists. The phenotypic data obtained from single-cell Raman microspectroscopy can be combined with the phylogenetic resolution of fluorescence in situ hybridization (FISH) to provide a powerful tool for investigating the structure and function of natural microbial communities (Raman-FISH). This chapter provides information about the use of isotopically labeled substrates to track isotope incorporation and hence specific metabolic processes within bacterial functional groups, through the use of Raman-FISH. A general overview of the equipment and techniques necessary for conducting Raman-FISH analysis is followed by a case study highlighting the utility of this technique for discovering the bacterial communities responsible for naphthalene degradation in groundwater microcosms.
Item Type: | Publication - Book Section |
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Digital Object Identifier (DOI): | https://doi.org/10.1007/978-3-540-77587-4 |
Programmes: | CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.1 - Interactions ... structure ecosystems and their functioning CEH Programmes pre-2009 publications > Biodiversity > BD03 The Genetic Basis of Ecological Function > BD03.1 Molecular ecology |
UKCEH and CEH Sections/Science Areas: | Hails |
ISBN: | 978-3-540-77588-1 |
Additional Keywords: | fluorescence in situ hybridization, raman spectroscopy, stable isotopes |
NORA Subject Terms: | Biology and Microbiology |
Related URLs: | |
Date made live: | 13 Oct 2010 13:19 +0 (UTC) |
URI: | https://nora.nerc.ac.uk/id/eprint/10623 |
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