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Cell lines from the soft tick Ornithodoros moubata

Bell-Sakyi, L.; Ruzek, D.; Gould, Ernest A.. 2009 Cell lines from the soft tick Ornithodoros moubata. Experimental and Applied Acarology, 49. 209-219. 10.1007/s10493-009-9258-y

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Abstract/Summary

Primary cell cultures (n = 16) were initiated from tissues of embryonic and neonatal larval Ornithodoros moubata following methods developed for hard ticks. After maintenance for 20–25 months in vitro, cell multiplication commenced in surviving cultures, leading to the establishment of six cell lines designated OME/CTVM21, 22, 24, 25, 26 and 27. All lines are maintained at 28°C, with subculture at 2–8 week intervals. The cultures comprise heterogeneous populations of large cells of 15–100 μm in diameter, often with finger-like protrusions and/or intracellular crystals, rarely attached, predominantly floating and forming clumps or hollow multicellular vesicles up to 1 mm in diameter. Attempts to cryopreserve the cells are described. Tick-borne encephalitis virus has been serially passaged ten times in OME/CTVM21 cells without significant decrease in virus production and with no change in its biological properties as shown by the size and morphology of plaques produced in porcine kidney cells.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1007/s10493-009-9258-y
Programmes: CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment
CEH Sections: Hails
ISSN: 0168-8162
Additional Information. Not used in RCUK Gateway to Research.: This is an Open Access paper and can be accessed via the Official URL link
Additional Keywords: Ornithodoros moubata, tick cell line, Tick-borne encephalitis virus
NORA Subject Terms: Biology and Microbiology
Date made live: 06 Apr 2010 09:30
URI: http://nora.nerc.ac.uk/id/eprint/9222

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