Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: a comparison of pooled and individual data

Talbot, Anita T.; Pottinger, Tom G.; Smith, Terry J.; Cairns, Michael T.. 2009 Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: a comparison of pooled and individual data. Fish and Shellfish Immunology, 27 (2). 309-317. https://doi.org/10.1016/j.fsi.2009.05.016

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Official URL: http://dx.doi.org/10.1016/j.fsi.2009.05.016

Abstract/Summary

This study set out to investigate whether differential expression of genes for acute phase proteins in rainbow trout (Oncorhynchus mykiss) could be induced by confinement stress, a non-invasive method of activating the neuroendocrine stress response. In addition, a second objective was to assess the variation in gene expression between individual fish within the population of stressed fish in an attempt to identify APP genes having uniform and consistent changes in expression during stress. The liver was chosen for this investigation as it is the primary site of acute phase protein synthesis. Relative expression of the eight genes including transferrin, fibrinogen-like protein 2 (flp2), α-1-anti-proteinase-like protein (α1-antiprot), leukocyte cell-derived chemotaxin 2 (LECT2), pentraxin, serum amyloid A (SAA), haptoglobin (Hp), and differentially regulated trout protein 1 (DRTP1) was analysed by quantitative real-time PCR (qPCR) over 5 experimental time points spanning the course of a week. The results showed that the expression of three genes, SAA, haptoglobin and DRTP1, were most altered as a result of exposure to confinement stress. A correlation was identified between the expression of haptoglobin and DRTP1. Gene expression analyses in individual fish found that the transcript levels of haptoglobin and DRTP1 genes varied much less between individuals than was the case for SAA. The increase of haptoglobin and DRTP1 gene expression and its uniformity in response to stress make these genes potential biomarkers for stress in trout.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.fsi.2009.05.016
Programmes:	CEH Programmes pre-2009 publications > Water > WA02 Quantifying processes that link water quality and quantity, biota and physical environment > WA02.4 Biological interactions
UKCEH and CEH Sections/Science Areas:	Parr
Additional Keywords:	stress, rainbow trout, acute phase protein, gene expression, cortisol
NORA Subject Terms:	Biology and Microbiology Zoology
Related URLs:	Author's Homepage
Date made live:	12 Nov 2009 16:31 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/7455

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1016/j.fsi.2009.05.016

Programmes:

CEH Programmes pre-2009 publications > Water > WA02 Quantifying processes that link water quality and quantity, biota and physical environment > WA02.4 Biological interactions

UKCEH and CEH Sections/Science Areas:

Parr

Additional Keywords:

stress, rainbow trout, acute phase protein, gene expression, cortisol

NORA Subject Terms:

Biology and Microbiology
Zoology