Human toenails as a biomarker of exposure to elevated environmental arsenic
Button, Mark; Jenkin, Gawen R.T.; Harrington, Chris F.; Watts, Michael J.. 2009 Human toenails as a biomarker of exposure to elevated environmental arsenic. Journal of Environmental Monitoring, 11 (3). 610-617. 10.1039/b817097eBefore downloading, please read NORA policies.
A pilot study was conducted to determine the applicability of toenails as a biomarker of exposure to elevated environmental arsenic (As) levels. A total of 17 individuals were recruited for the pilot study: 8 residents living near to a former As mine, Devon, UK, forming the exposed group, plus 9 residents from Nottinghamshire, UK, with no anticipated As exposure who were used for comparison as a control group. All toenail samples were thoroughly washed prior to analysis and the wash solutions retained for As determination via ICP-MS to provide an indication of the background environmental As levels for each group. Total As was determined in washed toenail samples via ICP-MS following microwave assisted acid digestion. Concentrations of total As in the toenails of the exposed group were elevated, ranging from 858 to 25981 µg kg-1 (geometric mean = 5406 µg kg-1), compared to the control group whose toenail As concentrations ranged from 73 to 273 µg kg-1 (geometric mean = 122 µg kg-1). Higher levels of exogenous As contamination were present on the toenails of the exposed group (geometric mean = 506 µg kg-1) compared to the control group (geometric mean = 4.0 µg kg-1) providing evidence of higher environmental As levels in the exposed group. Total As concentrations in toenail samples were positively correlated to environmental As levels (r = 0.60, p < 0.001). HPLC-ICP-MS analysis of aqueous toenail extracts revealed inorganic arsenite (AsIII) to be the dominant species extracted (83%) with lesser amounts of inorganic arsenate (AsV) and organic dimethylarsinate (DMAV) at 13% and 8.5%, respectively. Arsenic speciation in analysed toenail extracts from the two groups was comparable. The only notable difference between groups was the presence of small amounts (<1%) of organic methylarsonate (MAV) in two toenail samples from the exposed group. Toenails are presented as a viable biomarker of exposure at sites with elevated environmental As, such as the former mining sites found throughout Devon and Cornwall, UK.
|Item Type:||Publication - Article|
|Digital Object Identifier (DOI):||10.1039/b817097e|
|Programmes:||BGS Programmes 2009 > Environment and Health|
|Additional Keywords:||Arsenic, Health|
|NORA Subject Terms:||Earth Sciences|
|Date made live:||12 May 2009 12:39|
Actions (login required)