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Comparative sequence analysis of CP12, a small protein involved in the formation of a Calvin cycle complex in photosynthetic organisms

Groben, Rene; Kaloudas, Dimitrios; Raines, Christine A.; Offmann, Bernard; Maberly, Stephen C.; Gontero, Brigitte. 2010 Comparative sequence analysis of CP12, a small protein involved in the formation of a Calvin cycle complex in photosynthetic organisms. Photosynthesis Research, 103 (3). 183-194. 10.1007/s11120-010-9542-z

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Abstract/Summary

CP12, a small intrinsically unstructured protein, plays an important role in the regulation of the Calvin cycle by forming a complex with phosphoribulokinase (PRK) and glyceraldehyde-3- phosphate dehydrogenase (GAPDH). An extensive search in databases revealed 129 protein sequences from: higher plants, mosses and liverworts, different groups of eukaryotic algae and cyanobacteria. CP12 was identified throughout the Plantae, apart from in the Prasinophyceae. Within the Chromalveolata, two putative CP12 proteins have been found in the genomes of the diatom Thalassiosira pseudonana and the haptophyte Emiliania huxleyi, but specific searches in further chromalveolate genomes or EST datasets did not reveal any CP12 sequences in other Prymnesiophyceae, Dinophyceae or Pelagophyceae. A species from the Euglenophyceae within the Excavata also appeared to lack CP12. Phylogenetic analysis showed a clear separation into a number of higher taxonomic clades and among different forms of CP12 in higher plants. Cyanobacteria, Chlorophyceae, Rhodophyta & Glaucophyceae, Bryophyta, and the CP12-3 forms in higher plants all form separate clades. The degree of disorder of CP12 was higher in higher plants than in eukaryotic algae and cyanobacteria apart from the green algal class Mesostigmatophyceae that is ancestral to the streptophytes. This suggests that CP12 has evolved to become more flexible and possibly take on more general roles. Different features of the CP12 sequences in the different taxonomic groups and their potential functions and interactions in the Calvin cycle are discussed.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1007/s11120-010-9542-z
Programmes: CEH Topics & Objectives 2009 onwards > Water > WA Topic 2 - Ecohydrological Processes > WA - 2.3 - Assess the responses of river, lake and wetland ecosystems to ecohydrological drivers
CEH Topics & Objectives 2009 onwards > Water > WA Topic 2 - Ecohydrological Processes
CEH Sections: Parr
ISSN: 0166-8595
Additional Information. Not used in RCUK Gateway to Research.: For reprints please contact Rene Groben (rgro@ceh.ac.uk) or Brigitte Gontero (bmeunier@ifr88.cnrs-mrs.fr).
NORA Subject Terms: Botany
Biology and Microbiology
Date made live: 19 Apr 2010 10:33
URI: http://nora.nerc.ac.uk/id/eprint/7085

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