nerc.ac.uk

Single cell microbial ecophysiology with Raman-FISH

Read, Daniel; Huang, Wei E.; Whiteley, Andrew S.. 2016 Single cell microbial ecophysiology with Raman-FISH. In: McGenity, Terry J.; Timmis, Kenneth N.; Nogales, Balbina, (eds.) Hydrocarbon and lipid microbiology protocols: single-cell and single-molecule methods. Berlin, Springer, 65-76. (Springer Protocols Handbooks).

Full text not available from this repository.

Abstract/Summary

The ability to identify and characterise the roles that bacteria perform in their natural environment is a central prerequisite for understanding how ecosystems function. Traditional methods of culturing and identification are not always suitable due to the inability to grow most bacteria in pure cultures, the so-called great plate count anomaly. Recent developments in culture-independent molecular methods, coupled to microscopy-based ecophysiological analyses, are gaining increasing interest due to their ability to circumvent culture-based biases and allow physiological/phylogenetic analysis within ecological communities. Here we describe the application of Raman microspectroscopy and fluorescence in situ hybridisation (FISH) in combination with stable isotope labelling to help determine bacterial identity and function.

Item Type: Publication - Book Section
Digital Object Identifier (DOI): 10.1007/8623_2015_153
CEH Sections: Rees (from October 2014
ISBN: 9783662491294
Additional Keywords: FISH, fluorescent in situ hybridisation, Raman spectroscopy, stable isotope probing
NORA Subject Terms: Ecology and Environment
Biology and Microbiology
Date made live: 29 Jul 2016 13:13 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/514067

Actions (login required)

View Item View Item

Document Downloads

Downloads for past 30 days

Downloads per month over past year

More statistics for this item...