Unlocking the 'microbial black box' using RNA-based stable isotope probing technologies

Whiteley, Andrew S.; Manefield, Mike; Lueders, Tillmann. 2006 Unlocking the 'microbial black box' using RNA-based stable isotope probing technologies. Current Opinion in Biotechnology, 17 (1). 67-71. 10.1016/j.copbio.2005.11.002

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Microbial ecologists have long sought to associate the transformation of compounds in the environment with the microbial clades responsible. The development of stable isotope probing (SIP) has made this possible in many ecological and biotechnological contexts. RNA-based SIP technologies represent a significant leap forward for culture-independent ‘functional phylogeny’ analyses, where specific consumption of a given compound carrying a 13C signature can be associated with the small subunit ribosomal RNA molecules of the microbes that consume it. Recent advances have led to the unequivocal identification of microorganisms responsible for contaminant degradation in engineered systems, and to applications enhancing our understanding of carbon flow in terrestrial ecosystems.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1016/j.copbio.2005.11.002
Programmes: CEH Programmes pre-2009 publications > Biodiversity
CEH Sections: _ Molecular Microbial Ecology
ISSN: 0958-1669
Format Availability: Electronic, Print
NORA Subject Terms: Biology and Microbiology
Date made live: 15 Jun 2007 13:43

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