Unlocking the 'microbial black box' using RNA-based stable isotope probing technologies
Whiteley, Andrew S.; Manefield, Mike; Lueders, Tillmann. 2006 Unlocking the 'microbial black box' using RNA-based stable isotope probing technologies. Current Opinion in Biotechnology, 17 (1). 67-71. 10.1016/j.copbio.2005.11.002Full text not available from this repository.
Microbial ecologists have long sought to associate the transformation of compounds in the environment with the microbial clades responsible. The development of stable isotope probing (SIP) has made this possible in many ecological and biotechnological contexts. RNA-based SIP technologies represent a significant leap forward for culture-independent ‘functional phylogeny’ analyses, where specific consumption of a given compound carrying a 13C signature can be associated with the small subunit ribosomal RNA molecules of the microbes that consume it. Recent advances have led to the unequivocal identification of microorganisms responsible for contaminant degradation in engineered systems, and to applications enhancing our understanding of carbon flow in terrestrial ecosystems.
|Item Type:||Publication - Article|
|Digital Object Identifier (DOI):||10.1016/j.copbio.2005.11.002|
|Programmes:||CEH Programmes pre-2009 publications > Biodiversity|
|CEH Sections:||_ Molecular Microbial Ecology|
|Format Availability:||Electronic, Print|
|NORA Subject Terms:||Biology and Microbiology
|Date made live:||15 Jun 2007 13:43|
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