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Evaluation of flow cytometry as a method for quantification of circulating blood cell populations in salmonid fish

Morgan, J.A.W.; Pottinger, T.G.; Rippon, P.. 1993 Evaluation of flow cytometry as a method for quantification of circulating blood cell populations in salmonid fish. Journal of Fish Biology, 42 (1). 131-141. 10.1111/j.1095-8649.1993.tb00311.x

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Abstract/Summary

Flow cytometry was used to estimate the proportions of different blood cell types in brown and rainbow trout. On the basis of forward light scatter and 90° side scatter three populations were differentiated. The relative abundance of these cells correlated with that of erythrocytc (r2= 0.994), lymphocyte plus thrombocyte (r2= 0.676) and neutrophil populations (r2= 0.571) enumerated by direct microscopy. By density gradient separation of cells, cell sorting and acridine orange staining it was confirmed that these cell types could be assigned to the populations detected. Changes in blood cell populations were monitored by flow cytometry in a group of experimental fish placed under confinement stress. Flow cytometry proved to be a rapid and reliable method for monitoring cell population dynamics in fish blood.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1111/j.1095-8649.1993.tb00311.x
CEH Sections: _ Pre-2000 sections
ISSN: 0022-1112
Additional Keywords: brown trout, rainbow trout, erythrocyte, thrombocyte, neutrophil, lymphocyte, FSC, stress
NORA Subject Terms: Zoology
Biology and Microbiology
Date made live: 11 Aug 2015 08:07 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/511148

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