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Recombinant interleukin-1beta activates the hypothalamic–pituitary–interrenal axis in rainbow trout, Oncorhynchus mykiss

Holland, J.W.; Pottinger, T.G.; Secombes, C.J.. 2002 Recombinant interleukin-1beta activates the hypothalamic–pituitary–interrenal axis in rainbow trout, Oncorhynchus mykiss. Journal of Endocrinology, 175. 261-267. 10.1677/joe.0.1750261

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Abstract/Summary

The present study provides the first direct evidence that implicates fish cytokines as the effector molecules by which the immune system signals the neuroendocrine system and activates the hypothalamic–pituitary–interrenal stress axis. I.p. injections of trout recombinant interleukin-1beta (rIL-1beta) or E. coli lipopolysaccharide (LPS), at concentrations known to induce immune/inflammatory responses in vivo (0·1–0·6 nmol/kg and 1·3 mg/kg respectively), significantly elevated plasma cortisol levels in a dose- and/or time-dependent manner. However, in contrast to general stress responses in fish, under the conditions employed in this study, no specific treatment effects on plasma glucose levels could be demonstrated. The trout IL-1beta peptides (P1 and P3), which are homologous to receptor-binding sequences of human IL-1beta, failed to influence the prevailing cortisol concentration even though an equivalent dose has been found to have immunostimulatory properties in vivo. Blockade of endogenous ACTH release by administration of the synthetic glucocorticoid dexamethasone prevented the rIL-1beta/LPS-mediated elevation of plasma cortisol, suggesting that IL-1beta and LPS modulate cortisol secretion via effects at the level of the hypothalamic–pituitary axis. These data indicate that, with respect to IL-1beta, cytokine signalling between the immune and neuroendocrine systems in mammals appears to be conserved in lower vertebrates.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1677/joe.0.1750261
Programmes: CEH Programmes pre-2009 publications > Water
CEH Sections: _ Environmental Chemistry & Pollution
ISSN: 0022-0795
Additional Information. Not used in RCUK Gateway to Research.: Click on the Official URL link to access freely available full text.
Additional Keywords: interleukin-1beta, rainbow trout, hypothalamic-pituitary-interrenal axis, cortisol, intraperitoneal, stress
NORA Subject Terms: Zoology
Biology and Microbiology
Date made live: 23 Jun 2015 09:34 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/510784

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