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Molecular mechanisms of toxicity of silver nanoparticles in zebrafish embryos

van Aerle, Ronny; Lange, Anke; Moorhouse, Alex; Paszkiewicz, Konrad; Ball, Katie; Johnston, Blair D.; de-Bastos, Eliane; Booth, Timothy; Tyler, Charles R.; Santos, Eduarda M.. 2013 Molecular mechanisms of toxicity of silver nanoparticles in zebrafish embryos. Environmental Science & Technology, 47 (14). 8005-8014. 10.1021/es401758d

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Abstract/Summary

Silver nanoparticles cause toxicity in exposed organisms and are an environmental health concern. The mechanisms of silver nanoparticle toxicity, however, remain unclear. We examined the effects of exposure to silver in nano-, bulk-, and ionic forms on zebrafish embryos (Danio rerio) using a Next Generation Sequencing approach in an Illumina platform (High-Throughput SuperSAGE). Significant alterations in gene expression were found for all treatments and many of the gene pathways affected, most notably those associated with oxidative phosphorylation and protein synthesis, overlapped strongly between the three treatments indicating similar mechanisms of toxicity for the three forms of silver studied. Changes in oxidative phosphorylation indicated a down-regulation of this pathway at 24 h of exposure, but with a recovery at 48 h. This finding was consistent with a dose-dependent decrease in oxygen consumption at 24 h, but not at 48 h, following exposure to silver ions. Overall, our data provide support for the hypothesis that the toxicity caused by silver nanoparticles is principally associated with bioavailable silver ions in exposed zebrafish embryos. These findings are important in the evaluation of the risk that silver particles may pose to exposed vertebrate organisms.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1021/es401758d
Programmes: CEH Topics & Objectives 2009 - 2012 > Biogeochemistry > BGC Topic 3 - Managing Threats to Environment and Health > BGC - 3.2 - Provide the evidence base for setting Environment Quality Standards ...
CEH Topics & Objectives 2009 - 2012 > Biogeochemistry > BGC Topic 3 - Managing Threats to Environment and Health
CEH Topics & Objectives 2009 - 2012 > Biodiversity
CEH Sections: Acreman
ISSN: 0013-936X
NORA Subject Terms: Ecology and Environment
Biology and Microbiology
Date made live: 20 Mar 2014 11:13 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/506457

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