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Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts

Cook, Shelley; Chung, Betty Y.-W.; Bass, David; Moureau, Gregory; Tang, Shuoya; McAlister, Erica; Culverwell, C. Lorna; Glücksman, Edvard; Wang, Hui; Brown, T. David K.; Gould, Ernest A.; Harbach, Ralph E.; de Lamballerie, Xavier; Firth, Andrew E.. 2013 Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts. PLoS ONE, 8 (11), e80720. 22, pp. 10.1371/journal.pone.0080720

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Abstract/Summary

We investigated whether small RNA (sRNA) sequenced from field-collected mosquitoes and chironomids (Diptera) can be used as a proxy signature of viral prevalence within a range of species and viral groups, using sRNAs sequenced from wild-caught specimens, to inform total RNA deep sequencing of samples of particular interest. Using this strategy, we sequenced from adult Anopheles maculipennis s.l. mosquitoes the apparently nearly complete genome of one previously undescribed virus related to chronic bee paralysis virus, and, from a pool of Ochlerotatus caspius and Oc. detritus mosquitoes, a nearly complete entomobirnavirus genome. We also reconstructed long sequences (1503-6557 nt) related to at least nine further viruses. Crucially, several of the sequences detected were reconstructed from host organisms highly divergent from those in which related viruses have been previously isolated or discovered. It is clear that viral transmission and maintenance cycles in nature are likely to be significantly more complex and taxonomically diverse than previously expected.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1371/journal.pone.0080720
Programmes: CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.2 - Quantify the impact of invasive species, pathogens ...
CEH Sections: Reynard
ISSN: 1932-6203
Additional Information. Not used in RCUK Gateway to Research.: Open Access paper - Official URL link provides full text
NORA Subject Terms: Ecology and Environment
Biology and Microbiology
Date made live: 26 Nov 2013 16:07 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/503997

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