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A new medium for Caenorhabditis elegans toxicology and nanotoxicology studies designed to better reflect natural soil solution conditions

Tyne, William; Lofts, Stephen; Spurgeon, David J.; Jurkschat, Kerstin; Svendsen, Claus. 2013 A new medium for Caenorhabditis elegans toxicology and nanotoxicology studies designed to better reflect natural soil solution conditions. Environmental Toxicology and Chemistry, 32 (8). 1711-1717. 10.1002/Etc.2247

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Abstract/Summary

A new toxicity test medium for Caenorhabditis elegans is presented. The test solution is designed to provide a better representation of natural soil pore water conditions than currently available test media. The medium has a composition that can readily be modified to allow for studies of the influences of a range of environmentally relevant parameters on nematode biology and toxicology. Tests conducted in the new medium confirmed that nematodes' reproduction was possible at a range of solution pH levels, offering the potential to conduct toxicity studies under a variety of conditions. A test to establish silver nanoparticle and dissolved silver nitrate toxicity, a study type not feasible in M9 or agar media due to precipitation and nanoparticle agglomeration, indicated lower silver nanoparticle (median effective concentration [EC50] of 6.5 mg Ag/L) than silver nitrate (EC50 0.28 mg Ag/L) toxicity. Characterization identified stable nanoparticle behavior in the new test medium.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1002/Etc.2247
Programmes: CEH Topics & Objectives 2009 - 2012 > Biogeochemistry > BGC Topic 3 - Managing Threats to Environment and Health
CEH Sections: Acreman
Shore
ISSN: 0730-7268
Additional Keywords: nanoparticle, silver, toxicity test, ionic strength, fulvic acid, pH
NORA Subject Terms: Ecology and Environment
Date made live: 27 Sep 2013 15:03 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/503275

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