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Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR

Rhodes, Glenn; Fluri, Alexandra; Gerber, Marco; Henderson, Alan; Ruefenacht, Andrea; Pickup, Roger W.. 2008 Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR. Journal of Microbiological Methods, 73 (3). 266-268. 10.1016/j.mimet.2008.03.006

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Abstract/Summary

A quantitative real-time 5′-nuclease (Taqman) PCR technique was developed to specifically detect Mycobacterium immunogenum. rpoB-specific primers and Taqman probe were evaluated for detection of M. immunogenum DNA extracted from pure cultures and from industrial metal working fluids (MWFs). Specificity was confirmed and the sensitivity of detection of M. immunogenum genomic DNA was shown to be approximately 9 fg (2 cell equivalents). When tested on industrial metal working fluids from the UK and USA from which no M. immunogenum CFU were recovered, the assay detected between 3.4 × 101 and 1.9 × 104 cell equivalents (CE) per ml, and increased the detection rate over culture to 37.5% (12 of 32 samples). This assay provides a specific, sensitive and rapid method for the detection of M. immunogenum and is applicable within industry for the early detection of this human pathogen and to the possible prevention of hypersensitivity pneumonitis (HP) in workers.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1016/j.mimet.2008.03.006
Programmes: CEH Programmes pre-2009 publications > Biodiversity > BD02 An Integrated Framework for the Sustainable Management of Biological Introductions - Alien Species and Emerging Diseases
CEH Sections: Parr
ISSN: 0167-7012
Additional Keywords: Mycobacterium immunogenum, Mycobacterium chelonae complex, Hypersensitivity pneumonitis, Metal working fluids, Real-time PCR, Taqman
NORA Subject Terms: Biology and Microbiology
Health
Date made live: 26 Nov 2008 16:06
URI: http://nora.nerc.ac.uk/id/eprint/4811

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