Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells
Kelly, Barbara J.; King, Linda A.; Possee, Robert D.; Chappl, Susan D.J.. 2006 Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells. Journal of General Virology, 87. 531-536. 10.1099/vir.0.81312-0Full text not available from this repository.
Spodoptera frugiperda cells infected with Autographa californica nucleopolyhedrovirus (AcMNPV) lacking a functional anti-apoptotic p35 protein undergo apoptosis. However, such mutants replicate normally in Trichoplusia ni (TN-368) cells. An AcMNPV plaque isolate (AcdefrT) was identified during propagation of a virus deficient in p35 in TN-368 cells. This virus exhibited enhanced budded-particle formation in TN-368 cells, but was partially defective for polyhedra production in the same cells. Virus replication in AcdefrT-infected TN-368 cells was accompanied by extensive plasma-membrane blebbing and caspase activation late in infection, both features of apoptosis. Rescue of the p35 locus of AcdefrT continued to result in a reduction in polyhedra and increase in budded virus production in TN-368 cells, but no plasma-membrane blebbing was observed. The mutation was mapped to the FP-25 gene locus. This gene mutation combined with the non-functional p35 was found to be responsible for the cell-blebbing effect observed in AcdefrT-infected TN-368 cells.
|Item Type:||Publication - Article|
|Digital Object Identifier (DOI):||10.1099/vir.0.81312-0|
|Programmes:||CEH Programmes pre-2009 publications > Other|
|CEH Sections:||_ Molecular Virology & Parasitology|
|Format Availability:||Electronic, Print|
|NORA Subject Terms:||Biology and Microbiology|
|Date made live:||04 Jul 2007 11:33|
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