On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification

Tsaloglou, Maria-Nefeli; Bahi, Mahadji M.; Waugh, Edward M.; Morgan, Hywel; Mowlem, Matthew. 2011 On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification. Analytical Methods, 3. 2127-2133. 10.1039/C1AY05164D

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Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time RNA detection. The system is being developed to analyse the rbcL gene of phytoplankton Karenia brevis, a species responsible for harmful algal blooms. This integrated system will perform sub-cellular analysis of RNA using nucleic acid sequence-based amplification and would be used in large scale biochemical analysis and experimentation. The device could potentially be used for the detection of any species with a known target nucleic acid sequence for in situ environmental monitoring, forensics or clinical diagnostics.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1039/C1AY05164D
ISSN: 1759-9660
Date made live: 18 Aug 2011 08:56 +0 (UTC)

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