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Rapid characterisation of microbial biodegradation pathways by FT-IR spectroscopy

Huang, Wei E.; Hopper, David; Goodacre, Royston; Beckmann, Manfred; Singer, Andrew; Draper, John. 2006 Rapid characterisation of microbial biodegradation pathways by FT-IR spectroscopy. Journal of Microbiological Methods, 67. 273-280. 10.1016/j.mimet.2006.04.009

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Abstract/Summary

Fourier transform-infrared (FT-IR) spectroscopy has become an important tool for rapid analysis of complex biological samples. The infrared absorbance spectrum could be regarded as a “fingerprint” which is characteristic of biochemical substances. In this study, Pseudomonas putida NCIMB 9869 was grown with either 3,5-xylenol or m-cresol as the sole carbon source, each inducing different metabolic pathways for m-cresol biotransformation. FT-IR spectroscopy was capable of differentiating both induced cultures of P. putida NCIMB 9869 as well as the resulting biotransformation product mixtures. FT-IR spectral analysis indicated that carboxylic acids were key chemicals responsible for distinguishing the products of the two catabolic pathways. Gas chromatography–mass spectrometry (GC-MS) was performed to validate the FT-IR analysis, indicating that two carboxylic acids, 3-hydroxybenzoic acid and 2,5-dihydroxybenzoic acid, were present as m-cresol biotransformation products from 3,5-xylenol-grown cells, but were absent in m-cresol-grown cells. The ability to use FT-IR to rapidly distinguish between biotransformation product mixtures as well as differentially induced bacterial strains suggests this approach might be a valuable tool for screening large biotransformation assays for novel products and metabolic mutants.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1016/j.mimet.2006.04.009
Programmes: CEH Programmes pre-2009 publications > Biodiversity > SE01A Sustainable Monitoring and Management of Land Resources
CEH Sections: _ Environmental Biotechnology
ISSN: 0167-7012
Additional Keywords: FT-IR, metabolic profile, classification, biodegradation, pathway, genetic algorithm, Pseudomonas putida NCIMB 9869
NORA Subject Terms: Biology and Microbiology
Chemistry
Date made live: 02 May 2008 10:06
URI: http://nora.nerc.ac.uk/id/eprint/2872

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